- TC11-18H10.1 (See other available formats)
- Regulatory Status
- Other Names
- Interleukin-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
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IL-17, also known as CTLA-8, is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. IL-17 is produced by Th cells (Th17) that are distinct from the traditional Th1- and Th2-cell subsets. IL-23 plays an important role in triggering IL-17 production. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts, activation of NF-κB, and costimulation of T cell proliferation. IL-17 is an essential inflammatory mediator in the development of autoimmune diseases. Neutralization of IL-17 with monoclonal antibody is able to ameliorate the disease course.Product Details
- Antibody Type
- Host Species
- E. coli expressed, recombinant mouse IL-17A
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Red Laser (633 nm)
- Application Notes
ELISA Capture3,4 and ELISPOT Capture5: The purified TC11-18H10.1 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated TC11-8H4 antibody (Cat. No. 507002) as the detecting antibody and recombinant mouse IL-17 (Cat. No. 576009) as the standard.
Flow Cytometry2-4,7,8,11,12: The TC11-18H10.1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-17-producing cells within mixed cell populations.
Neutralization6,9: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-17 bioactivity in vivo and in vitro (Cat. No. 506906).
(PubMed link indicates BioLegend citation)
- Kennedy J, et al. 1996. J. Interferon Cytokine Res. 16:611.
- Schubert D, et al. 2004. J. Immunol. 172:4503. (ICFC)
- Infante-Duarte C, et al. 2000. J. Immunol. 165:6107. (ICFC, ELISA Capture)
- Harrington LE, et al. 2005. Nature Immunol. doi:10.1038/ni1254. (ICFC, ELISA Capture)
- Nekrasova T, et al. 2005. J. Immunol. 175:2734. (ELISPOT Capture)
- Yen D, et al. 2006. J. Clin. Invest. 116:1310. (Neut)
- Ehirchiou D, et al. 2007. J. Exp. Med. 204:1519. (ICFC)
- Kang SG, et al. 2007. J. Immunol. 179:3724. (ICFC)
- Smith E, et al. 2008. J. Immunol. 181:1357. (Neut) PubMed
- Neufert C, et al. 2007. Eur. J. Immunol. 37:1809. PubMed
- Wang C, et al. 2009. Mucosal Immunol 2:173. (ICFC) PubMed
- Cui Y, et al. 2009. Invest. Ophth. Vis. Sci. 50:5811. (ICFC) PubMed
- Kivisäkk P, et al. 2009. Ann. Neurol. 65:457. PubMed
- Cooney LA, et al. 2011. J. Immunol. 187:4440. PubMed
- Ma Y, et al. 2012. PLoS One. 7:e40763. PubMed
- Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
- Product Citations
AB_536013 (BioLegend Cat. No. 506911)
AB_536014 (BioLegend Cat. No. 506912)
- Cytokine; dimer; 15 kD (Mammalian).
- Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
- Cell Sources
- CD4+ memory T cells
- Cell Targets
- Fibroblasts, epithelial and endothelial cells, stromal cells
- IL-17R (CD217)
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Antigen References
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Numasaki M, et al. 2002. Blood 101:2620.
3. Fossiez F, et al. 1996. J. Exp. Med. 183:2593.
4. Yao Z, et al. 1997. Cytokine 9:794.
5. Dong C. 2006. Nat. Rev. Immunol. 6:329.
6. Hofstetter HH, et al. 2005 Cell. Immunol. 237:123.
- Gene ID
- 16171 View all products for this Gene ID
- View information about IL-17A on UniProt.org
- Does the anti-mouse IL-17 antibody (clone TC11-18H10.1) recognize the A or F isoform?
- Clone TC11-18H10.1 recognizes IL-17A isoform, but it also recognizes the IL-17A/F heterodimer via IL-17A binding.
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