Alexa Fluor® 647 anti-mouse/human CD282 (TLR2) Recombinant Antibody

Pricing & Availability
Clone
QA16A01 (See other available formats)
Regulatory Status
RUO
Other Names
Toll-like receptor 2, TLR-2, TIL4, CD282, TLR2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA16A01_A647_CD282_Antibody_021218
Human peripheral blood monocytes were stained with Alexa Fluor® 647 anti-mouse/human CD282 (clone QA16A01, filled histogram) or Alexa Fluor® 647 mouse IgG1, κ isotype control (open histogram).
  • QA16A01_A647_CD282_Antibody_021218
    Human peripheral blood monocytes were stained with Alexa Fluor® 647 anti-mouse/human CD282 (clone QA16A01, filled histogram) or Alexa Fluor® 647 mouse IgG1, κ isotype control (open histogram).
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
153007 25 µg $130.00
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153008 100 µg $275.00
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Description

CD282 (Toll-like receptor 2, TLR2) is an 85kDa, type I transmembrane protein, with 14 leucine-rich repeats (LRR) in the extracellular domain and a toll/IL-1R (TIR) in the cytoplasmic region. TLR2 is expressed by monocytes/macrophages, dendritic cells, granulocytes, endothelial cell and epithelial cells. TLR2 associates with TLR1 and TLR6 in the cell surface and is involved in the recognition of pathogen-associated molecular patterns (PAMPs) such as peptidoglycan, lipoteihoic acid (LTA), lipoarabinomanan, lipoproteins, and LPS from certain Gram-negative bacteria, yeast, spirochete and fungi.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Human
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Recombinant extracellular domain of murine TLR2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Blocking of IL-6 and TNF-a production induced by P3CSK4 on RAW264.7 cells was observed during development.

Product Citations
  1. Korin B, et al. 2020. Sleep. :43. PubMed
RRID
AB_2728207 (BioLegend Cat. No. 153007)
AB_2728208 (BioLegend Cat. No. 153008)

Antigen Details

Structure
Type I transmembrane protein, 14 leucine-rich repeats (LRR) in the extracellular domain, the intracellular domain contains a toll/IL-1R (TIR) domain.
Distribution

Monocytes/macrophages, dendritic cells, granulocytes, endothelial cell, epithelial cells.

Function
Activates innate host defense, induces production of pro-inflammatory cytokines.
Interaction
TLR1, TLR6 , MYD88 and TRAF6.
Ligand/Receptor
Peptidoglycan, lipoteihoic acid (LTA), lipoarabinomanan, lipoproteins, as well as different LPS from certain Gram-negative bacteria, yeast, spirochete and fungi.
Cell Type
Dendritic cells, Endothelial cells, Epithelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
CD Molecules, Toll Like Receptors
Antigen References

1. Biswas S, et al. 2016. Blood. 127:2618.
2. Wang Y, et al. 2016. Proc. Natl. Acad. Sci. USA 113:884.
3. Ramstead AG, et al. 2016. Infect. Immun. 84:940.
4. Scheeren FA, et al. 2014. Nat. Cell. Biol. 16:1238.
5. Barbalat R, et al. 2009. Nat. Immunol. 10:1200. 

Gene ID
24088 View all products for this Gene ID 7097 View all products for this Gene ID
UniProt
View information about CD282 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 02/12/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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