- 1B11 (See other available formats)
- Regulatory Status
- Other Names
- Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, gp115
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD43, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, and gp115, is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. Due to variable glycosylation and sialylation, two isoforms of CD43 have been identified. The 115 kD glycoform of CD43 is expressed on most hematopietic cells including T lymphocytes, NK cells, monocytes, granulocytes, platelets, and CD5+ B cells. It is not present on resting B cells and erythrocytes. While the 130 kD glycoform is thought to be activation-associated form primarily expressed on myeloid cells, pre-B cells, and activated T cells. It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion, as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors. The 1B11 antibody reacts with the activation-associated glycoform of CD43. The epitope recognized by 1B11 is also shared with desialylated CD45RB. This antibody is useful for differentiation of effector CD8 T cells and memory T cells.Product Details
- Antibody Type
- Host Species
- Mouse WEHI 274.3 myeloid tumor cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
IHC-F - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,2, and immunohistochemistry of acetone-fixed frozen sections.
(PubMed link indicates BioLegend citation)
- Jones AT, et al. 1994. J. Immunol. 153:3426. (IP, WB)
- Carlow DA, et al. 1999. J. Immunol. 163:1441. (IP, WB)
- Onami TM, et al. 2002. J. Immunol. 168:6022.
- van der Most RG, et al. 2003. Intl. Immunol. 15:119.
- Chu VT, et al. 2007. J. Immunol. 179:5947.
- Lang A, et al. 2008. J. Immunol. 180:4848. PubMed
- Gibbert K, et al. 2010. J. Immunol. 185:6179. PubMed
- Product Citations
AB_528804 (BioLegend Cat. No. 121212)
- Single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites, 115 kD and 130 kD glycoforms with different glycosylation and sialylation
Most hematopoietic cells including peripheral T cells, NK cells, granulocytes, monocytes, thymocytes, platelets, plasma cells, CD5+ B-1 cells and activated B cells, and hemotopoietic progenitor cells.
- Play a dual roles in cell-cell adhesion and anti-adhesion, costimulation of cell activation and survival, induce apoptosis of T cells and hematopoietic progenitors
- CD54, Siglec-1
- Cell Type
- T cells, NK cells, Granulocytes, Monocytes, Thymocytes, Platelets, Plasma cells, B cells, Hematopoietic stem and progenitors
- Biology Area
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. van den Berg TK, et al. 2001. J. Immunol. 166:3637.
2. Moore T, et al. 1994. J. Immunol. 153:4978.
3. Onami TM, et al. 2002. J. Immunol. 168:6022.
4. Tong J, et al. 2004. J. Exp. Med. 199:1277.
5. Jones AT, et al. 1994. J. Immunol. 153:3426.
- Gene ID
- 20737 View all products for this Gene ID
- View information about CD43 on UniProt.org
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