Alexa Fluor® 647 anti-mouse CD200 (OX2) Antibody

Pricing & Availability
OX-90 (See other available formats)
Regulatory Status
Other Names
Rat IgG2a, κ
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Product Citations
C57BL/6 mouse splenocytes were stained with CD19 Brilliant Violet 421™ and CD200 (clone OX-90) Alexa Fluor® 647 (left), or rat IgG2a, κ Alexa Fluor® 647 isotype control (left).
  • 1-OX-90_A647_CD200_Antibody_010620
    C57BL/6 mouse splenocytes were stained with CD19 Brilliant Violet 421™ and CD200 (clone OX-90) Alexa Fluor® 647 (left), or rat IgG2a, κ Alexa Fluor® 647 isotype control (left).
  • 2-OX-90_A647_CD200_Antibody_101819
    C57 frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then the section was stained with 5 µg/mL of CD3 (clone 17A2) Alexa Fluor® 594 (green) and CD200 (clone OX-90) Alexa Fluor® 647 (red) at 4°C overnight. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X object and stitched with MetaMorph® software.
See Alexa Fluor® 647 spectral data
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123815 25 µg $95.00
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123816 100 µg $245.00
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CD200 (OX-2 antigen) is a type-1 membrane glycoprotein containing two extracellular Ig-like domains. CD200 a highly conserved type I membrane glycoprotein that is expressed on a variety of cell types including thymocytes, some T cells, endothelial and follicular dendritc cells, B cells, and brain tissue (neurons); but not on NK cells, granulocytes, monocytes, or macrophages. CD200 costimulates T cell proliferation. It may regulate myeloid cell activity in a variety of tissues. CD200 is the ligand for CD200 receptor (CD200R). The CD200 Receptor is restricted to myeloid cells, and it is believed that its engagement with CD200 results in inhibition and/or downregulation of myeloid cell activity. Blocking of CD200/CD200R interactions decreases myeloid cell inhibitory thresholds which results in enhanced immune activation.

Product Details
Technical data sheet

Product Details

Antibody Type
Host Species
Soluble fusion protein of the extracellular region of mouse OX-2 antigen with domains 3 and 4 of rat CD4 fusion protein.
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested
IHC-F - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
AB_2819849 (BioLegend Cat. No. 123815)
AB_2819850 (BioLegend Cat. No. 123816)

Antigen Details

Immunoglobulin (Ig) superfamily

Thymocytes, some T cells, endothelial, B cells

Co-stimulatory factor in T cell proliferation
Antigen References

1. Hoek RM, et al. 2000. Science 290:1768.
2. Gorczynski R, et al. 2004. J. of Immunol. 172:7744.
3. Gorczynski L, et al. 1999. J. Immunol. 162:774.
4. Rosenblum MD, et al. 2004. Blood 103:2691.
5. Zhang S, et al. 2004. J. of Immunol. 173:6786.
6. Barclay AN, et al. 2002. Trends Immunol. 23:285.

Gene ID
4345 View all products for this Gene ID
View information about CD200 on

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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