Alexa Fluor® 647 anti-mouse CD182 (CXCR2) Antibody

Pricing & Availability
Clone
SA044G4 (See other available formats)
Regulatory Status
RUO
Other Names
IL-8RB, GRO/MGSA, Cmkar2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
SA044G4_AF647_CXCR2_Antibody_FC_1_051315
C57BL/6 mouse bone marrow cells were stained with Ly-6G/Ly-6C PE and CXCR2 (clone SA044G4) Alexa Fluor® 647 (top) or rat IgG2a, κ Alexa Fluor® 647 isotype control (bottom).
  • SA044G4_AF647_CXCR2_Antibody_FC_1_051315
    C57BL/6 mouse bone marrow cells were stained with Ly-6G/Ly-6C PE and CXCR2 (clone SA044G4) Alexa Fluor® 647 (top) or rat IgG2a, κ Alexa Fluor® 647 isotype control (bottom).
  • SA044G4_AF647_CXCR2_Antibody_FC_2_051315
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
149305 25 µg $125.00
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149306 100 µg $325.00
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Description

CD182, also known as CXCR2, is a G-protein coupled receptor with 7 transmembrane regions that is involved in chemotaxis, neutrophil activation and angiogenesis. CXCR2 is expressed by neutrophils, basophils, subset of T cells, monocytes, macrophages, NK and NKT cells. Its ligands are CXCL1, CXCL2, CXCL3 and CXCL5.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CXCR2-transfected cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Product Citations
  1. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  2. Girbl T et al. 2018. Immunity. 49(6):1062-1076 . PubMed
  3. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  4. Barkaway A, et al. 2021. Immunity. . PubMed
RRID
AB_2565693 (BioLegend Cat. No. 149305)
AB_2565694 (BioLegend Cat. No. 149306)

Antigen Details

Structure
G-protein coupled receptor, 7 transmembrane regions.
Distribution

Neutrophils, basophils, subset of T cells, monocytes, macrophages, and natural killer cells (NK).

Function
Chemotaxis, neutrophil activation, and angiogenesis
Ligand/Receptor
CXCL1, CXCL2, CXCL3, and CXCL5.
Cell Type
Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Spaan AN, et al. 2014. Nat. Commun. 5:5438.
2. Natsuaki Y, et al. 2014. Nat. Immunol. 11:1064.
3. Hsieh CY, et al. 2014. J. Immunol. 193:3693.
4. Diana J, and Lehuen A. 2014. EMBO Mol. Med. 6:1090.
5. Stoolman JS, et al. 2014. J. Immunol. 193:564.

Gene ID
12765 View all products for this Gene ID
UniProt
View information about CD182 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/18/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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