Alexa Fluor® 647 anti-mouse CD144 (VE-cadherin) Antibody

Pricing & Availability
Clone
BV13 (See other available formats)
Regulatory Status
RUO
Other Names
Vascular endothelial-cadherin
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
BV13_AF647_042710
Mouse endothelial cells b.End.3 stained with BV13 Alexa Fluor® 647
  • BV13_AF647_042710
    Mouse endothelial cells b.End.3 stained with BV13 Alexa Fluor® 647
See Alexa Fluor® 647 spectral data
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138005 25 µg $150.00
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138006 100 µg $350.00
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Description

CD144, also known as vascular endothelial-cadherin (VE-cadherin), is a 120 kD member of the type II Cadherin family.  It is an endothelial specific hemophilic adhesion molecule involved in endothelial cell survival, migration, contact-dependent growth inhibition, and homophilic adhesion.  VE-cadherin is essential for maintaining the integrity of the endothelial barrier in vivo.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
VE-cadherin-Ig fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Clone BV13 recognizes an epitope between aa 45 and 56, and has a binding affinity of 5-15 nM5. Additional reported applications (for relevant formats) include: Western blotting1, blocking of cell interactions in vivo1, and immunofluorescence microscopy staining4.

Application References

(PubMed link indicates BioLegend citation)
  1. Corada M, et al. 1999. P. Natl. Acad. Sci. USA 96:9815. (WB, Block)
  2. Liao F, et al. 2000. Cancer Res. 60:6805. (FC)
  3. Crosby CV, et al. 2005. Blood 105:2771. (FC)
  4. Liao F, et al. 2002. Cancer Res. 62:2567. (IF)
  5. May C, et al. 2005. Blood 105:4337. (epitope)
Product Citations
  1. Kedem A, et al. 2017. J Ovarian Res. 10.1186/s13048-017-0354-z. PubMed
  2. Xu C, et al. 2018. Nat Commun. 9:2449. PubMed
  3. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  4. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  5. Pinho S et al. 2018. Developmental cell. 44(5):634-641 . PubMed
  6. Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
  7. Himburg HA et al. 2018. Cell stem cell. 23(3):370-381 . PubMed
  8. Dravis C et al. 2018. Cancer cell. 34(3):466-482 . PubMed
  9. Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
  10. Baryawno N et al. 2019. Cell. 177(7):1915-1932 . PubMed
  11. Himburg HA, et al. 2019. J Clin Invest. 130:315. PubMed
  12. Rybtsov S, et al. 2011. J Exp Med. 208:1305. PubMed
  13. Gordon-Keylock S, et al. 2013. Blood. 122:2338. PubMed
  14. Renner B, et al. 2013. J Am Soc Nephrol. 24:1849. PubMed
  15. Mizoguchi T, et al. 2014. Dev Cell. 29:340. PubMed
  16. Kostadinova E, et al. 2016. Sci Rep. 6:30943. PubMed
  17. Himburg H, et al. 2016. Nat Microbiol. 2:16232. PubMed
  18. Poulos M, et al. 2016. Nat Commun. 7:13829. PubMed
  19. Asada N, et al. 2017. Nat Cell Biol. 19:214-223. PubMed
  20. Lehmann GL, et al. 2020. J Exp Med. 217:00:00. PubMed
RRID
AB_10568319 (BioLegend Cat. No. 138005)
AB_10569114 (BioLegend Cat. No. 138006)

Antigen Details

Structure
A 120 kD member of type II Cadherin family
Distribution

Endothelial cells

Function
Involved in endothelial cell survival, migration, contact-dependent growth inhibition, and hemophilic adhesion
Ligand/Receptor
CD144
Cell Type
Endothelial cells, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Allport JR, et al. 2002. J. Leukocyte Biol. 71:821.
2. Hirashima M, et al. 2009. Blood 93:1253.
3. Matsuyoshi N, et al. 1997. Proc. Assoc. Am. Physicians 109:362.
4. Matsumura K, et al. 2003. Blood 101:1367.
5. Hirashima M, et al. 2009. Blood 101:2261.
6. Gotsch U, et al. 1997. J. Cell Sci. 110:583.
7. Kataoka H, et al. 1997. Dev. Growth Differ. 39:729.

Gene ID
12562 View all products for this Gene ID
UniProt
View information about CD144 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 01/12/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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