- M3/84 (See other available formats)
- Regulatory Status
- Other Names
- CD107b, LAMP-2
- Rat IgG1, κ
- Ave. Rating
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- Product Citations
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Mac-3 is a 110 kD type I membrane glycoprotein, also known as CD107b and LAMP-2. It is expressed on lysosomal membranes and the plasma membrane of macrophages and some myeloid cell lines. In the bone marrow, few cells display Mac-3 antigen on the surface, but a large proportion express Mac-3 in the cytoplasm. CD107b has been identified as a ligand for galaptin, an S-type lectin present in the extracellular matrix. Mac-3/CD107b is upregulated in some tumors and increased expression has been correlated with enhanced metastatic potential.Product Details
- Antibody Type
- Host Species
- Membrane glycoproteins from C57BL/6 mouse peritoneal exudate cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
ICC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. For immunocytochemistry, a concentration range of 5.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1-4 and immunohistochemical staining of acetone-fixed frozen sections5,6 and paraformaldehyde-fixed paraffin-embedded sections9-11.
(PubMed link indicates BioLegend citation)
- Springer TA. 1981. J. Biol. Chem. 256:3833. (IP)
- Ho MK, et al. 1983. J. Biol. Chem. 258:636. (IP)
- Chen JW, et al. 1985. J. Cell Biol. 101:85. (IP)
- Ralph P, et al. 1983. J. Immunol. 130:108. (IP)
- Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC)
- Kano M, et al. 1998. Transplantation 65:837. (IHC)
- Terrazas LI,et al.2005. Int J Parasitol. 35:1349. PubMed
- Hayashida A, et al. 2011. J. Biol Chem. 286:3288. PubMed
- Vollmar P, et al. 2010. J. Immunol. 185:6338. (IHC)
- Odorisio T, et al. 2002. J. Cell Sci. 115:2559. (IHC)
- Nessler S, et al. 2007. Brain 130:2186. (IHC)
- Product Citations
AB_493311 (BioLegend Cat. No. 108511)
AB_493310 (BioLegend Cat. No. 108512)
- 110 kD
Macrophages, some myeloid cell lines
- Differentiation and activation
- Cell Type
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
1. Springer TA. 1981. J. Biol. Chem. 256:3833.
2. Ho MK, et al. 1983. J. Biol. Chem. 258:636.
3. Ralph P, et al. 1983. J. Immunol. 130:108.
- Gene ID
- 16784 View all products for this Gene ID
- View information about CD107b on UniProt.org
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