- 3C4 (MIC2/4) (See other available formats)
- Regulatory Status
- Other Names
- Rat IgG2a, κ
- Ave. Rating
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- Product Citations
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CD102 is a 55 kD immunoglobulin superfamily member, also known as ICAM-2. CD102 is a heavily glycosylated cell surface ligand for LFA-1 (CD11a/CD18). CD102 is constitutively expressed on vascular endothelial cells, some lymphocytes, monocytes, and platelets. CD102 is thought to be involved in lymphocyte recirculation and T cell activation. The 3C4(MIC2/4) antibody can block interactions between ICAM-2 and LFA-1.Product Details
- Antibody Type
- Host Species
- COS cells transfected with mouse ICAM-2 cDNA
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining2 of acetone-fixed frozen sections, in vitro blocking of ligand binding1, and immunoprecipitation1. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105608).
(PubMed link indicates BioLegend citation)
- Xu H, et al. 1996. J. Immunol. 156:4909.
- Gerwin N, et al. 1999. Immunity 10:9.
- Product Citations
AB_2122183 (BioLegend Cat. No. 105611)
AB_2122182 (BioLegend Cat. No. 105612)
- Ig superfamily, 55 kD
Vascular endothelial cells, some resting lymphocytes, monocytes, platelets
- Lymphocyte recirculation, costimulation
- LFA-1, Mac-1
- Cell Type
- Endothelial cells, Lymphocytes, Monocytes, Platelets
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Casasnovas JM, et al. 1999. P. Natl. Acad. Sci. USA 96:3017.
3. Diamond MS, et al. 1990. J. Cell Biol. 111:3129.
- Gene ID
- 15896 View all products for this Gene ID
- View information about CD102 on UniProt.org
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