Alexa Fluor® 647 anti-human CD294 (CRTH2) Antibody

Pricing & Availability
BM16 (See other available formats)
Regulatory Status
Other Names
Chemoattractant receptor-homologous molecule expressed on Th2 cells, GPR44, G-protein coupled receptor 44, DP2
Rat IgG2a, κ
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Product Citations
Human peripheral blood lymphocytes stained with CCR4 PerCP/Cy5.5 and BM16 Alexa Fluor® 647 (top) or rat IgG2a Alexa Fluor® 647 isotype control (bottom) (gated on CD4+ lymphocyte population)
  • BM16_AF647_1_081910
    Human peripheral blood lymphocytes stained with CCR4 PerCP/Cy5.5 and BM16 Alexa Fluor® 647 (top) or rat IgG2a Alexa Fluor® 647 isotype control (bottom) (gated on CD4+ lymphocyte population)
  • BM16_AF647_2_081910
  • BM16_AF647_3_081910
    Human Th2 polarized CD4+ T cells stained with BM16 Alexa Fluor® 647
Compare all formats See Alexa Fluor® 647 spectral data
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350103 25 tests $90.00
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350104 100 tests $240.00
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CD294, also known as CRTH2, is a seven-transmembrane protein coupled with heterotrimeric G proteins. CRTH2 is the prostaglandin D2 receptor and is expressed by Th2 cells, eosinophils, and basophils. CD294 prevents the apoptosis of Th2 cells and mediates the chemotaxis of CRTH2 expressing cells to the sites of allergic inflammation, such as the asthmatic lung.

Product Details
Technical data sheet

Product Details

Antibody Type
Host Species
Rat cell line TART/B19-12.10 transfected with human CRTH2
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

This product is sold under license agreement with BML, Inc for research use only.

Application References

(PubMed link indicates BioLegend citation)
  1. Nagata K, et al. 1999. J. Immunol. 162:1278. (FC)
  2. De Fanis U, et al. 2007. Blood 109:4343. (FC)
  3. Tsuda H, et al. 2001. Clin. Exp. Immunol. 123:105. (FC)
  4. Hamada K, et al. 2004. Allergology International 53:179. (FC)
Product Citations
  1. Wright AKA, et al. 2017. J Allergy Clin Immunol. 140:1430. PubMed
AB_10640066 (BioLegend Cat. No. 350103)
AB_10642025 (BioLegend Cat. No. 350104)

Antigen Details

Seven-transmembrane protein coupled with G proteins

Effector T helper 2 (Th2) cells, eosinophils, basophils

Chemotaxis of eosinophils, basophils and Th2 cells, prevention of apoptosis in Th2 cells
Heterotrimeric G proteins
Prostaglandin D2
Cell Type
Th2, Eosinophils, Basophils
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family
CD Molecules
Antigen References

1. Luster AD and Tager AM. 2004. Nat. Rev. Immunol. 4:711.
2. Monneret G, et al. 2001. Blood 98:1942.
3. Xue L, et al. 2009. J. Immunol. 182:7580.
4. Schratl P, et al. 2007. J. Immunol. 179:4792.

Gene ID
11251 View all products for this Gene ID
View information about CD294 on

Related FAQs

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Go To Top Version: 2    Revision Date: 06/27/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD294 (CRTH2)

  • Alexa Fluor® 647 anti-human CD294 (CRTH2)

  • PE anti-human CD294 (CRTH2)

  • FITC anti-human CD294 (CRTH2)

  • APC anti-human CD294 (CRTH2)

  • Brilliant Violet 421™ anti-human CD294 (CRTH2)

  • APC/Cyanine7 anti-human CD294 (CRTH2)

  • PerCP/Cyanine5.5 anti-human CD294 (CRTH2)

  • PE/Cyanine7 anti-human CD294 (CRTH2)

  • Brilliant Violet 510™ anti-human CD294 (CRTH2)

  • Brilliant Violet 605™ anti-human CD294 (CRTH2)

  • Brilliant Violet 711™ anti-human CD294 (CRTH2)

  • PE/Dazzle™ 594 anti-human CD294 (CRTH2)

  • TotalSeq™-A0102 anti-human CD294 (CRTH2)

  • Pacific Blue™ anti-human CD294 (CRTH2)

  • TotalSeq™-C0102 anti-human CD294 (CRTH2)

  • APC/Fire™ 750 anti-human CD294 (CRTH2) Antibody

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