Alexa Fluor® 594 anti-mouse Podoplanin Antibody

Pricing & Availability
Clone
PMab-1 (See other available formats)
Regulatory Status
RUO
Other Names
T1a, gp38, stromal cell marker
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
PMab-1_A594_Podoplanin_Antibody_1_012319
C57 mouse frozen kidney section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then the section was stained with 10 µg/mL of Alexa Fluor® 488 anti-mouse CD326/EpCAM (clone G8.8, green), 10 µg/mL of Alexa Fluor® 594 anti-mouse podoplanin (clone PMab-1, red) at 4°C overnight. Nuclei was counter stained with DAPI (blue). The image was scanned with a 10X object and stitched with MetaMorph® software.
  • PMab-1_A594_Podoplanin_Antibody_1_012319
    C57 mouse frozen kidney section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then the section was stained with 10 µg/mL of Alexa Fluor® 488 anti-mouse CD326/EpCAM (clone G8.8, green), 10 µg/mL of Alexa Fluor® 594 anti-mouse podoplanin (clone PMab-1, red) at 4°C overnight. Nuclei was counter stained with DAPI (blue). The image was scanned with a 10X object and stitched with MetaMorph® software.
  • PMab-1_A594_Podoplanin_Antibody_2_050819
    Formalin-fixed, 300 micron-thick mouse kidney section was blocked, permeabilized and stained overnight with CD31 (clone MEC13.3) Alexa Fluor® 647 (red) at 5 µg/mL, Tubulin Beta 3 (TUBB3)(clone AA10) Alexa Fluor® 488 (green) at 2.5 µg/mL, and Podoplanin (clone Pmab-1) Alexa Fluor® 594 (blue) at 5 µg/mL, optically cleared, then analyzed at 230 µm imaging depth on a confocal microscope. Watch the video.
  • PMab-1_A594_Podoplanin_Antibody_3_3D-IHC_092121.png
    Paraformaldehyde-fixed (4%), 500 μm-thick mouse kidney section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was costained with anti-mouse Podoplanin Antibody (clone PMab-1) Alexa Fluor® 594 at 5 µg/mL (green), and anti-mouse CD31 Antibody (clone MEC13.3) Alexa Fluor® 647 at 5 µg/mL (magenta) and counterstained with DAPI (blue). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 20X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
    Watch the video.
See Alexa Fluor® 594 spectral data
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156205 25 µg $145.00
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156206 100 µg $295.00
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Description

The mucin-type glycoprotein podoplanin is thought to be involved in the development of the lymphatic vascular system. Podoplanin is named after its expression in the kidney glomerular epithelial cells (podocytes). It has a potential role in tumor progression.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Peptide (38-51 amino acids of mouse podoplanin)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested
3D IHC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 5.0 - 10 µg/mL is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application References

(PubMed link indicates BioLegend citation)
  1. Yamada S, et al. 2017. Monoclon Antib Immunodiagn Immunother. 36(2):77-79.
RRID
AB_2800698 (BioLegend Cat. No. 156205)
AB_2819915 (BioLegend Cat. No. 156206)

Antigen Details

Structure
43 kD glycosylated type-1 transmembrane protein. Mucin-type protein.
Distribution

Expressed on epithelial and mesothelial cells.

Function
Mediates effects on cell migration and adhesion through its different partners. During development plays a role in blood and lymphatic vessels separation by binding CLEC1B, triggering CLEC1B activation in platelets and leading to platelet activation and/or aggregation.
Interaction
Interacts with CLEC1B; the interaction is independent of CLEC1B glycosylation and activates CLEC1B; the interaction is dependent of sialic acid on O-glycans.
Ligand/Receptor
CLEC1B
Cell Type
Epithelial cells
Biology Area
Cancer Biomarkers, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Antigen References
  1. Farr A, et al. 1992. J Histochem Cytochem. 40:651.
  2. Schacht V, et al. 2005. Am J Pathol. 166:913.
Gene ID
14726 View all products for this Gene ID
UniProt
View information about Podoplanin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 09/21/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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