- BMA-12 (See other available formats)
- Regulatory Status
- Other Names
- Neuroimmune semaphorin 4D, Sema4D, M-Sema G, Semaphorin-C-like 2, Semaphorin-J, Semaj, Semcl2, Coll-4, Semacl2
- Rat IgG2a, κ
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- Product Citations
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CD100, also known as Semaphorin-4D (SEMA4D), is a 150 kD, type I transmembrane protein, with one sema domain and one C2-type Ig domain. It is found as a homodimer on the cell surface. CD100 is expressed in the brain, kidney, heart, the majority of hematopoietic cells, and some malignant cells. CD100 is a potent inducer of angiogenesis and inhibitor of bone formation, and plays a role in lymphocyte activation, antibody production, dendritic cell maturation, and axon guidance. Its ligands are CD72 and Plexin-B1. CD100 is cleaved from the membrane of activated T and B cells, producing a soluble form.Product Details
- Antibody Type
- Host Species
- Mouse CD100-Fc fusion protein
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
IHC-F - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescence staining. For immunohistochemistry, a concentration range of 5-10 μg/ml is suggested. For immunofluorescence microscopy, a concentration range of 5-10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Application Notes
Additional reported applications for the relevant formats include: immunoprecipitation from cell lysates2, capture antibody function in a sandwich ELISA assay2, and immunohistochemical staining of frozen brain sections4.
(PubMed link indicates BioLegend citation)
- Kumanogoh A, et al. 2000. Immunity. 13:621. (FC)
- Wang X, et al. 2001. Blood. 97:3498. (FC, IP, ELISA)
- Kumanogoh A, et al. 2002. J. Immunol. 169:1175. (FC)
- Moreau-Fauvarque C, et al. 2003 J. Neurosci. 23:9229. (IHC)
- Product Citations
AB_2687303 (BioLegend Cat. No. 147609)
AB_2687304 (BioLegend Cat. No. 147610)
- Type I transmembrane protein, one sema domain, one C2-type immunoglobulin domain, homodimer, 150 kD.
Highly expressed on most hematopoietic cells, is also found in the brain, kidney, and heart. Overexpressed by some malignant cell types.
- Potent inducer of angiogenesis, inhibitor of bone formation, plays a role in lymphocyte activation, antibody production and dendritic cell maturation. In neurons, regulates dendrite branching and axon guidance.
- CD72, Plexin-B1.
- Cell Type
- B cells, Dendritic cells, Neutrophils, Tregs
- Biology Area
- Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Signal Transduction, Synaptic Biology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell. 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.
- Gene ID
- 20354 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- Specificity Alt (DOES NOT SHOW ON TDS):
- View information about CD100 on UniProt.org
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