Alexa Fluor® 594 anti-human CD144 (VE-Cadherin) Antibody

Pricing & Availability
Clone
BV9 (See other available formats)
Regulatory Status
RUO
Other Names
VE-Cadherin, cadherin-5, CDH5
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1_BV9_A594_CD144_ICC_032618
Human umbilical vein endothelial cells (HUVEC) were fixed with 1% paraformaldehyde (PFA) and blocked with 5% FBS for 30 minutes. Then the cells were stained with 10 µg/ml anti-human CD144 (clone BV9) Alexa Fluor® 594 (red). Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
  • 1_BV9_A594_CD144_ICC_032618
    Human umbilical vein endothelial cells (HUVEC) were fixed with 1% paraformaldehyde (PFA) and blocked with 5% FBS for 30 minutes. Then the cells were stained with 10 µg/ml anti-human CD144 (clone BV9) Alexa Fluor® 594 (red). Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
  • 2_BV9_A594_CD144_IHCP_032618
    Human paraffin-embedded placenta tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for 5 minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of anti-human CD144 (clone BV9) Alexa Fluor® 594 at 4°C overnight. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
See Alexa Fluor® 594 spectral data
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348512 100 µg $275.00
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Description

CD144, also known as VE-cadherin and cadherin-5, is a 140 kD glycoprotein which is composed of five extracellular cadherin repeats and a highly conserved cytoplasmic tail region. It is a calcium-dependent transmembrane cell-cell adhesion molecule localized at the intercellular boundaries of endothelial cells, hematopoietic stem cells, and perineurial cells. It functions as a classic cadherin by mediating homophilic adhesion and functions as a plasma membrane attachment site for the cytoskeleton. CD144 is thought to play a role in vascular development, permeability, and remodeling.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
IHC-P - Verified 

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5.0 - 10 μg/ml is recommended. For immunohistochemistry, a concentration range of 5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Clone BV9 has been shown to block VE-cadherin, causing a redistribution of VE-cadherin away from intracellular junctions.6 This clone binds to EC3-EC4 region in the extracellular domain of human VE-cadherin.7 Additional reported applications (for the relevant formats) include: Western Blotting1,2, immunofluorescence microscopy1,3, immunoprecipitation1,4, blocking angiogenesis in vitro4,5, inhibiting VE-cadherin reorganization4, and inducing endothelial cell apoptosis4.

Application References

(PubMed link indicates BioLegend citation)
  1. Almagro S, et al. 2010. Mol. Cell Biol. 30:1703. (WB, IF, IP)
  2. Zhang F, et al. 2004. J. Biol. Chem. 279:11760. (WB)
  3. Iurlaro M, et al. 2004. Am. J. Pathol. 165:181. (IF)
  4. Corada M, et al. 2001. Blood 97:1679. (IP, Block)
  5. Kooistra M, et al. 2005. FEBS 579:4966. (Block)
  6. Corada M, et al. 2001. Blood 97:1679. (Block)
  7. Bouillet L, et al. 2013. Laboratory Investigation 93:1194-11202.
RRID
AB_2563354 (BioLegend Cat. No. 348512)

Antigen Details

Structure
Member of the cadherin family; calcium-dependent transmembrane cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats and a highly conserved cytoplasmic tail region
Distribution

Endothelial cells, hematopoietic stem cells, perineurial cells

Function
Mediates calcium-dependent homophilic cell adhesion, plays fundamental roles in microvascular permeability and in the morphogenic and proliferative events associated with angiogenesis
Ligand/Receptor
VE-cadherin
Cell Type
Endothelial cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase
Antigen References

1. Taddei A, et al. 2008. Nat. Cell Biol. 10:923.
2. Gavard J, et al. 2006. Nat. Cell Biol. 8:1223.
3. Kim I, et al. 2005. Blood 106:903.
4. Suzuki S, et al. 1991. Cell Regul. 2:261.

Gene ID
1003 View all products for this Gene ID
UniProt
View information about CD144 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 05/04/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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