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Alexa Fluor® 555 Goat anti-rat IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4054 (See other available formats)
Regulatory Status
RUO
Isotype
Goat Polyclonal IgG
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Product Citations
publications
1_Poly4054_AF555_IgG_Antibody_072021.png
NIH/3T3 mouse fibroblasts were fixed with 1% paraformaldehyde and blocked with 5% FBS for 30 minutes. Then the cells were stained with 10 µg/mL purified anti-mouse/human CD44 (clone IM7) at room temperature for three hours, followed by 2.5 µg/mL anti-rat IgG (clone Poly4054) Alexa Fluor® 555 (red). Nuclei were counterstained with DAPI (blue). The image was captured with a 20X objective.
  • 1_Poly4054_AF555_IgG_Antibody_072021.png
    NIH/3T3 mouse fibroblasts were fixed with 1% paraformaldehyde and blocked with 5% FBS for 30 minutes. Then the cells were stained with 10 µg/mL purified anti-mouse/human CD44 (clone IM7) at room temperature for three hours, followed by 2.5 µg/mL anti-rat IgG (clone Poly4054) Alexa Fluor® 555 (red). Nuclei were counterstained with DAPI (blue). The image was captured with a 20X objective.
  • 2-Poly4054_AF555_IgG_Antibody_091118
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the section was stained with 10 µg/mL of Purified anti-mouse/human CD45R/B220 (clone RA3-6B2) and anti-mouse CD3ε (clone 145-2C11) Alexa Fluor® 647 (red) overnight at 4°C, followed by 2.5 µg/mL of anti-rat IgG (clone Poly4054) Alexa Fluor® 555 (green) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
Compare all formats See Alexa Fluor® 555 spectral data
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405420 100 µg $188
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Description

Goat anti-rat IgG antibody reacts primarily with the heavy chains of rat IgG, but also partially binds to the light chains common to most rat immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rabbit, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other immunoglobulins from other species.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Polyclonal
Host Species
Goat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 555 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 555 has an excitation maximum of 555 nm, and a maximum emission of 565 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This goat anti-rat IgG antibody is useful for direct immunofluorescent staining or as a second step reagent for indirect immunofluorescent staining when used in conjunction with primary antibodies.

Application References

(PubMed link indicates BioLegend citation)
  1. Zornetzer GA, et al. 2010. J. Virol. 84:11297. PubMed
  2. Frank NY, et al. 2011. Cancer Res. 71:1474. (IHC) PubMed
  3. Baba T, et al. 2020. eLife. 9:e54257. (IHC-P)
Product Citations
  1. Challa DK, et al. 2019. MAbs. 11:848. PubMed
  2. Luo L, et al. 2021. J Neuroinflammation. 18:27. PubMed
  3. Kurashima Y, et al. 2021. Nat Commun. 12:1067. PubMed
RRID
AB_2563180 (BioLegend Cat. No. 405420)

Related FAQs

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Go To Top Version: 5    Revision Date: 07/20/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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