- 1F4 (See other available formats)
- Regulatory Status
- Other Names
- T cell antigen receptor complex, T3
- Mouse IgM, κ
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- Product Citations
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CD3 is a complex composed of δ, γ, ε, and ζ chains. They are 20-25 kD members of the immunoglobulin superfamily and associated with the T cell receptor (TCR). CD3 is expressed on thymocytes, peripheral T cells, some NK-T cells, and dendritic epidermal T cells. CD3 is involved in antigen recognition, signal transduction, and T cell activation.Product Details
- Antibody Type
- Host Species
- F344 rat spleen cells stimulated with PMA and calcium ionophore
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
IHC-F - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. For immunohistochemistry staining on frozen tissue sections, a concentration range of 2.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Application Notes
Immobilized 1F4 antibody can induce T cell proliferation in vitro. Additional reported applications (for relevant formats of this clone) include: immunohistochemistry of acetone-fixed frozen sections1 and formaldehyde- fixed paraffin embedded sections4,5 immunofluorescence microscopy3, in vivo activation of T cell responses1, and in vivo inhibition of T cell responses2.
(PubMed link indicates BioLegend citation)
- Tanaka T, et al. 1989. J. Immunol. 142:2791. (Activ, IHC, IP)
- Nicholls MR, et al. 1993. Transplantation 55:459. (Block)
- Elbe A, et al. 1993. J. Invest. Dermatol. 102:74. (IF)
- Baba T, et al. 2006. Blood 107:2004. (IHC)
- Fujishiro J, et al. 2010. Am. J. Transplant. 10:1545-55. (IHC-P)
- Li X, et al. 2009. J. Immunol. 183:3955. (FC) PubMed
AB_893303 (BioLegend Cat. No. 201406)
- Ig superfamily, approximately 20-25 kD
Thymocytes, peripheral T cells, dendritic epidermal T cells, NK-T cells
- Antigen recognition, TCR signal transduction, T cell activation
- Peptide antigen/MHC complex
- Cell Type
- NKT cells, T cells, Thymocytes
- Biology Area
- Molecular Family
- CD Molecules
- Antigen References
1. Tanaka T, et al. 1989 J. Immunol. 142:2791.
2. Elbe A, et al. 1993. J. Invest. Dermatol. 102:74.
- Gene ID
- 25710 View all products for this Gene ID 300678 View all products for this Gene ID 315609 View all products for this Gene ID 25300 View all products for this Gene ID
- View information about CD3 on UniProt.org
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