- SMI 52 (See other available formats)
- Regulatory Status
- Other Names
- Microtubule-associated protein 2, MAP-2
- Mouse IgG1
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- Product Citations
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Microtubules are 25nm diameter protein rods found in most kinds of eukaryote cells. They are polymerized from a dimeric subunit made of one α subunit and one β tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein τ (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5.Product Details
- Mouse, Rat
- Antibody Type
- Host Species
- This monoclonal antibody was raised against Microtubule-Associated Protein 2 (MAP2).
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
IHC-P - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Application Notes
Additional reported applications (for relevant formats) include: immunocytochemistry5,6, ELISA7, and WB1,5.
SMI 52 reacts with microtubule-associated protein 2 (MAP2). This antibody reacts with most mammalian species and with frog tissue. SMI 52 recognizes neuronal cell bodies and dendrites in tissue sections and cell cultures. In immunoblots of brain cytoskeletal preparations, SMI 52 reacts with MAP2a and b, and also with a doublet at 68 kD that may represent MAP2c.
(PubMed link indicates BioLegend citation)
- Shi Y, et al. 2017. Cell Death Differ. 24(1):167. (WB)
- Shelton MA, et al. 2015. Biol. Psychiatry. 78(6):374. (IHC) PubMed
- Wang X, et al. 2015.PLOS ONE. 10: 0145441. (IHC) PubMed
- Kazim SF, et al. 2014. Neuro Dis. 71:110. (IHC)
- Cardenas-Aguayo MC, et al. 2013. PLOS ONE. (1):e53596. (ICC, WB) PubMed
- Lo Furno D, et al. 2013. J. Cell. Physiol. 228:2109. (ICC)
- Gensel JC, et al. 2009.J. Neursci 29: 3956-3968. (ELISA) PubMed
AB_2721365 (BioLegend Cat. No. 801804)
AB_2721366 (BioLegend Cat. No. 801805)
- MAP2 is made up of two bands of an apparent molecular weight of approximately 280 kD, referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kD on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternative transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein τ (tau).
Cellular distribution: Plasma membrane, cytoskeleton, nucleus, and cytosol.
Tissue distribution: Predominantly in the nervous system
- MAP2 encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are involved in microtubule assembly. The products of these genes are neuron-specific cytoskeletal proteins that are enriched in dendrites, which implicate a role in determining and stabilizing dentritic shape during neuron development.
- MAP2 interacts with microtubules. It has also been reported to interact with KNDC1 via KIND2.
- MAP2 isoforms are expressed in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites. In contrast τ (tau) is found predominantly in neuronal axons.
- Cell Type
- Neural Stem Cells
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- Antigen References
- Zhou Y, et al. 2015, Neuro Oncol. 17(12):1578.
- Loeser H, et al. 2015, Am J Nephrol. 41(3):191.
- Gene ID
- 17756 View all products for this Gene ID 25595 View all products for this Gene ID
- View information about MAP2 on UniProt.org
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