Alexa Fluor® 488 anti-human/rat CD51 (Integrin αvβ5) Antibody

Pricing & Availability
Clone
P1F6 (See other available formats)
Regulatory Status
RUO
Other Names
ITGAV, MSK8, VNRA, VTNR
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P1F6_A488_CD51_Antibody_090716
Human fibrocarcinoma cell line HT1080 was stained with CD51 (clone P1F6) Alexa Fluor® 488 (closed histogram) or mouse IgG1, κ Alexa Fluor® 488 isotype control (open histogram).
  • P1F6_A488_CD51_Antibody_090716
    Human fibrocarcinoma cell line HT1080 was stained with CD51 (clone P1F6) Alexa Fluor® 488 (closed histogram) or mouse IgG1, κ Alexa Fluor® 488 isotype control (open histogram).
See Alexa Fluor® 488 spectral data
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920009 25 tests $105.00
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920010 100 tests $225.00
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Description

CD51 is a type I integral membrane glycoprotein, known as vitronectin receptor α chain, or integrin αV. It forms heterodimer with integrin β1 (CD29), β3 (CD61), β5, β6, or β8. CD51 contains two disulfide-linked subunits of 125 kD and 24 kD, and is expressed on endothelial cells, fibroblasts, macrophages, platelets, osteoclasts, neuroblastoma, melanoma, and hepatoma cells. Many extracellular matrix proteins with RGD-motifs are CD51 ligands. In association with its β chains, CD51 binds vitronectin, von Willebrand factor, fibronectin, thrombospondin, osteopontin, fibrinogen, and laminin. CD51, as an adhesion molecule, plays important roles in leukocytes homing and rolling and mediates bone absorption and angiogenesis.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Rat, Cross-Reactivity: Hamster
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
The P1F6 monoclonal antibody was generated against human small cell lung carcinoma cells (UCLA P3).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

P1F6, reacts with the avß5 integrin complex. This antibody does not cross react with any other av containing integrin and completely inhibits avß5 dependent binding to vitronectin coated surfaces.

Product Citations
  1. André P et al. 2018. Cell. 175(7):1731-1743 . PubMed
RRID
AB_2629781 (BioLegend Cat. No. 920009)
AB_2629782 (BioLegend Cat. No. 920010)

Antigen Details

Structure
Two disulfide-linked subunits of 125 kD and 24 kD.
Distribution

Endothelial cells, fibroblasts, macrophages, platelets, osteoclasts, neuroblastoma, melanoma, and hepatoma cells.

Function
As an adhesion molecule, it plays important roles in leukocytes homing and rolling as well as mediates bone absorption and angiogenesis.
Interaction
Forms heterodimer with integrin β1 (CD29), β3 (CD61), β5, β6, or β8.
Cell Type
Endothelial cells, Fibroblasts, Macrophages, Mesenchymal Stem Cells, Osteoclasts, Platelets
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Burvenich I, et al. 2008. Mol. Cancer Ther. 7(12):3771.
2. Lee P, et al. 2014. J. Biol. Chem. 289(3):1467.
3. Su G, et al. 2007. Am. J. Respir. Cell Mol. Biol. 36(3):377.
4. Wayner EA, et al. 2007. Methods in Enzymology 426:117.
5. Weinacker A, et al. 1994. J. Biol. Chem. 269(9):6940.

Gene ID
3685 View all products for this Gene ID
UniProt
View information about CD51 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/08/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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