Alexa Fluor® 488 anti-human CD18 Antibody

Pricing & Availability
Clone
1B4/CD18 (See other available formats)
Regulatory Status
RUO
Other Names
Integrin beta-2, integrin beta chain, beta 2
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1B4slashCD18_A488_CD18_Antibody_082517
Human peripheral blood lymphocytes were stained with CD18 (clone 1B4/CD18) Alexa Fluor® 488 (filled histogram) or mouse IgG2a, κ Alexa Fluor® 488 isotype control (open histogram).
  • 1B4slashCD18_A488_CD18_Antibody_082517
    Human peripheral blood lymphocytes were stained with CD18 (clone 1B4/CD18) Alexa Fluor® 488 (filled histogram) or mouse IgG2a, κ Alexa Fluor® 488 isotype control (open histogram).
See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Check Availability Save
373403 25 tests $125.00
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373404 100 tests $275.00
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Description

CD18 is a 90-95 kD type I transmembrane protein also known as integrin β2 subunit, LFA-1 β subunit, and β2 integrin. CD18 non-covalently associates with CD11a, CD11b or CD11c. CD18 is expressed on all leukocytes. CD18 and associated α chains function in adhesion and signaling in hematopoietic cells.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human peripheral blood lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

The 1B4/CD18 antibody was able to partially block the Ts1/18 antibody, indicating the two anti-CD18 antibodies recognize distinct but overlapping epitopes. 

Application References

(PubMed link indicates BioLegend citation)
  1. Singer II, et al. 1993. J. Immunol. 150:2844.
  2. Seth R, et al. 1991. FEBS Lett. 282:193.
RRID
AB_2716019 (BioLegend Cat. No. 373403)
AB_2716020 (BioLegend Cat. No. 373404)

Antigen Details

Structure
Integrin, type I transmembrane glycoprotein
Distribution

Leukocytes

Function
Adhesion and signaling in hematopoietic cells
Interaction
Associates with CD11a, CD11b, or CD11c
Ligand/Receptor
ICAMs
Cell Type
Leukocytes, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Hogg N, et al. 1999. J. Clin. Invest. 103:97.
2. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175.
3. Springer TA. 1994. Cell 76:301.

Gene ID
3689 View all products for this Gene ID
UniProt
View information about CD18 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 08/29/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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