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Veri-Cells™ Leukocytes

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Regulatory Status
RUO
Other Names
Control cells
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Veri-Cells_Leukocytes_1_12212016
Veri-Cells™ Leukocytes were stained with anti-human CD19 (clone HIB19) APC, CD16 (clone 3G8) PE and CD56 (clone HCD56) PE surface markers or CD4 Brilliant Violet 421™ and FoxP3 (clone 206D) PE. Above: Veri-Cells™ Leukocytes scatter pattern. Below: T-cell, B-cell, Natural Killer cell and FoxP3 staining profiles.
  • Veri-Cells_Leukocytes_1_12212016
    Veri-Cells™ Leukocytes were stained with anti-human CD19 (clone HIB19) APC, CD16 (clone 3G8) PE and CD56 (clone HCD56) PE surface markers or CD4 Brilliant Violet 421™ and FoxP3 (clone 206D) PE. Above: Veri-Cells™ Leukocytes scatter pattern. Below: T-cell, B-cell, Natural Killer cell and FoxP3 staining profiles.
  • Veri-Cells_Leukocytes_2_12212016
  • Veri-Cells_Leukocytes_3_12212016
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426003 10 tests $112
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426001 25 tests $176
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426004 50 tests $364
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426002 100 tests $586
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Description

The reconstituted lyophilized human leukocytes can be used for immunofluorescent staining and multi-color flow cytometry assays.   The cells can be used as controls for stability studies to avoid donor variability. These cells have been verified to work with commonly tested cell surface markers such as CD3, CD4, CD8, CD19, CD20, CD56/16, CD14, CD25, CD69, HLA-DR, Granzyme B, Perforin, Foxp3, and Helios.

View all 100+ markers verified by LEGENDScreen™.

This product contains human cells, a potentially biohazardous material. Blood used in preparation of these samples was tested and found to be negative, using FDA approved methods, for antibody and nucleic acid testing against Human Immunodeficiency virus (HIV), Hepatitis B and C virus and HTLV (Human T-Lymphotrophic virus I and II), surface antigen for Hepatitis B (HBsAg) virus and syphilis and West Nile Virus. Biological tests are not 100% accurate. Use standard precautions when handling and treat as if the product is capable of transmitting disease. When handling or disposing, follow precautions described in CDC and FDA recommendations and OSHA Bloodborne Pathogen guidelines.

Product Details
Technical data sheet

Kit Contents

Kit Contents

Cat. No. 426001

  • 1 vial of lyophilized Veri-Cells™Leukocytes (4-6 x 106 cells)
  • 1 vial containing 1.5 ml of Veri-Cells™ Buffer A Plus

Cat. No. 426002

  • 4 vials of lyophilized Veri-Cells™ Leukocytes (4-6 x 106 cells per vial)
  • 4 vials containing 1.5 ml of Veri-Cells™ Buffer A Plus each

Cat. No. 426003

  • 2 vials of lyophilized Veri-Cells™ Leukocytes (> 1 x 106 cells per vial)
  • 1 vial containing 700 µl of Veri-Cells™ Buffer A Plus each

Cat. No. 426004

  • 10 vials of lyophilized Veri-Cells™ Leukocytes (> 1 x 106 cells per vial)
  • 5 vial containing 700 µl of Veri-Cells™ Buffer A Plus each
     

Veri-Cells™ Buffer A Plus is an improved version of the original Veri-Cells™ Buffer A to improve staining performance with tandem dyes.

Product Details

Formulation
Cat. Nos. 426003 and 426004: ≥ 1 X 106 lyophilized human peripheral blood leukocytes per vial.
Cat. Nos. 426001 and 426002: ≥ 5 X 106 lyophilized human peripheral blood leukocytes per vial.
Preparation
The Veri-Cells™ Leukocyte Kit is prepared from lyophilized human leukocytes and a vial of Veri-Cells™ Buffer A Plus.
Storage & Handling
Store the kit at 2°C - 8°C upon receipt. Do not open sealed pouch until ready to use. Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided. Contact BioLegend Technical Support for information on the current lots.
Application

FC - Quality tested
ICFC - Verified
Recommended Usage

50 µl per 5 test vials (after reconstitution with 325 µl)
50 µl per 25 test vials (after reconstitution with 1.3 ml)

Note: Veri-Cells™ Buffer A Plus may show precipitation over time, however this is normal and does not affect performance of the buffer.

Application Notes

The Veri-Cells™ Leukocytes Kit can be used as a positive control for surface and intracellular immunofluorescent staining and multi-color flow cytometry assays. It can also be used for the detection of intracellular molecules such as Granzyme B, Perforin, Foxp3 and Helios using BioLegend’s Fixation Buffer (Cat. No. 420801) and Intracellular Staining Permeabilization Wash Buffer (10X) (Cat. No. 421002) or the True Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401). For best results, Cell Staining Buffer (Cat. No. 420201) is recommended for any washing steps. Please note, any claims relative to product performance are based on testing with BioLegend reagents and protocols. Deviations from the use of these reagents and procedures have not been assessed and may affect performance.
 

Cell subset

Acceptance Criteria, % of lymphocyte gate

CD3+

55-95

CD4+CD3+

30-65

CD8+CD3+

10-40

CD19+CD3 –

3-30

CD56+CD16+CD3 –

3-40

CD14+

2-20

CD15+

50-90


Note on indicated ranges: Percent positive values are based on gates set on unstained cells. The antibodies used for testing were the following: CD56 PE (clone 5.1H11), CD16 PE (clone 3G8), CD19 APC (clone HIB19), CD3 FITC (clone UCHT1), CD4 PE/Cy7 (clone RPA-T4), CD8 APC/Cy7 (clone RPA-T8), CD14 (clone HCD14) PE/Dazzle™ 594 and CD15 (clone W6D3) Pacific Blue™. Variability in antibodies used, instruments, staining conditions and other factors may influence end-user values. The Certificate of Analysis specific values are provided as a reference. It is recommended that individual laboratories establish its own control values and ranges for each parameter based on laboratory specific conditions and protocols.

Please use our CoA Look-Up Tool to find the expected lot-specific frequencies of certain cell populations included within each lot of Veri-Cells™ Leukocytes. The antibodies used for testing were the following: CD56 PE (clone 5.1H11), CD16 PE (clone 3G8), CD19 APC (clone HIB19), CD3 FITC (clone UCHT1), CD4 PE/Cy7 (clone RPA-T4), CD8 APC/Cy7 (clone RPA-T8), CD14 PE/Dazzle™ 594 (clone HCD14) and CD15 Pacific Blue™ (clone W6D3). Percent positive is based on gates set on unstained cells. The Certificate of Analysis specific values are provided only as a reference. Variability in antibodies used, instruments, staining conditions and other factors may influence end-user values. It is recommended that individual laboratories establish its own control values and ranges for each parameter based on laboratory specific conditions and protocols.

Additional Product Notes

Veri-Cells™ Buffer A Plus is an improved version of the original Veri-Cells™ Buffer A to improve staining performance with tandem dyes.

Antigen Details

Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
NA

Related FAQs

Which markers can be stained?

Veri-Cells™ products have been validated against the human LEGENDScreen™ panel. View all the cell markers verified here.

Go To Top Version: 4    Revision Date: 06/03/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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