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Veri-Cells™ Activated (Cytokine) PBMC

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Regulatory Status
RUO
Other Names
Control Cells
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VeriCells_Acivated_Cytokine_PBMC_1_102618
Veri-Cells™ Activated (Cytokine) PBMC stained with CD3 FITC and TNF-α PerCP/Cyanine5.5, or GM-CSF Pacific Blue™ (left). Plots were gated based on corresponding isotype control stains (right).
  • VeriCells_Acivated_Cytokine_PBMC_1_102618
    Veri-Cells™ Activated (Cytokine) PBMC stained with CD3 FITC and TNF-α PerCP/Cyanine5.5, or GM-CSF Pacific Blue™ (left). Plots were gated based on corresponding isotype control stains (right).
  • VeriCells_Acivated_Cytokine_PBMC_2_102618
    Veri-Cells™ Activated (Cytokine) PBMC stained with CD3 FITC and IL-17a Brilliant Violet™ 421, or IFNγ APC (left). Plots were gated based on corresponding isotype control stains (right).
  • VeriCells_Acivated_Cytokine_PBMC_3_102618
    Veri-Cells™ Activated (Cytokine) PBMC stained with CD3 FITC and IL-2 APC/Cy7, or IL-4 PE/Cy7 (left). Plots were gated based on corresponding isotype control stains (right).
  • VeriCells_Acivated_Cytokine_PBMC_4_102618
    Veri-Cells™ Activated (Cytokine) PBMC stained with CD3 FITC and IL-8 APC, or IL-13 APC (left). Plots were gated based on corresponding isotype control stains (right).
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427001 10 tests $147
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427003 50 tests $528
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Description

The reconstituted Veri-Cells™ Activated (Cytokine) PBMC can be used for immunofluorescent staining and multi-color flow cytometry assays. The cells can be used as controls for cytokine staining with minimal variability across experiments. These cells have been verified to work with commonly tested cell surface (CD3, CD4, CD8, CD19, CD56) as well as cytokine markers such as IL-2, IL-3, IL-4, IL-5, IL-8, IL-13, IL-17, GM-CSF, IFN gamma, and TNF alpha. Some targets such as IL-5 are rare and will require the acquisition of at least 100,000 CD3+ events.

This product contains human cells, a potentially biohazardous material. Blood used in preparation of these samples was tested and found to be negative for antibody and nucleic acid testing against Human Immunodeficiency virus (HIV), Hepatitis B and C virus and HTLV (Human T-Lymphotrophic virus I and II), surface antigen for Hepatitis B (HBsAg) virus and syphilis and West Nile Virus using FDA approved methods. Biological tests are not 100% accurate. Use standard precautions when handling, and treat as if the product is capable of transmitting disease. When handling or disposing, follow precautions described in CDC and FDA recommendations and OSHA Bloodborne Pathogen guidelines.

Product Details
Technical data sheet

Kit Contents

Kit Contents

Cat. No. 427001

  • 2 vials of Veri-Cell™ Activated (Cytokine) PBMC (≥1 X 106 cells per vial) 
  • 1 vial of 0.7 ml Veri-Cells™ Buffer A Plus

Cat. No. 427003

  • 10 vials of Veri-Cell™ Activated (Cytokine) PBMC (≥1 X 106 cells/vial)
  • 5 vials of 0.7 ml Veri-Cells™ Buffer A Plus
     

Veri-Cells™ Buffer A Plus is an improved version of the original Veri-Cells™ Buffer A to improve staining performance with tandem dyes.

Product Details

Formulation
Cat. No. 427001: 2 vials each containing ≥ 1 X 106 lyophilized human peripheral blood mononuclear cells (PBMC).
Cat. No. 427003: 10 vials each containing ≥ 1 X 106 lyophilized human peripheral blood mononuclear cells (PBMC).
Preparation
The Veri-Cells™ Activated (Cytokine) PBMC kit is prepared from 2 vials of lyophilized human activated PBMCs and a vial of Veri-Cells™ Buffer A Plus. Veri-Cells Activated (Cytokine) PBMC contain lyophilized PBMCs that had been stimulated using Cell Activation Cocktail with Brefeldin A (Cat. No. 423303) for 6 hours.
Storage & Handling
Store the kit at 2°C – 8°C upon receipt. Do not open sealed pouch until ready to use. Once reconstituted, the cells may be used for up to five days if properly stored at 2°C – 8°C in the buffer provided.
Application

FC - Quality tested
Recommended Usage

50 µl per test (after reconstitution with 325 µl of Veri-Cells™ Buffer A Plus)

Note: Veri-Cells™ Buffer A Plus may show precipitation over time, however this is normal and does not affect performance of the buffer.

Application Notes

Veri-Cells™ Activated (Cytokine) PBMC Kit can be used as a positive control for detecting common surface (CD3, CD4, CD8, CD19, CD56) as well as cytokine markers such as IL-2, IL-3, IL-4, IL-5, IL-8, IL-13, IL-17a, GM-CSF, IFN gamma, and TNF alpha. Assessing the suitability of using this product as a control for end user’s molecule of interest is recommended, as each lot is verified based on the expression levels of markers mentioned above. For best results, Cell Staining Buffer is recommended for any washing steps. Product performance specifications are based on testing with BioLegend reagents and protocols. Deviations from the use of these reagents and procedures have not been assessed and may affect performance.

Antibodies Used for Product Validation

Target

Clone

Fluorophore

CD3

SK7

FITC

IL-13

JES10-5A2

PE/Cy7

GM-CSF

BVD2-21C11

Pacific Blue™

TNF-alpha

Mab11

PerCP/Cy5.5

IL-2

MQ1-17H12

APC/Cy7

IL-8

E8N1

APC

IL-17a

BL168

Brilliant Violet 421™

IFN-gamma

4S.B3

APC

IL-5

TRFK5

APC

IL-4

MP4-25D2

PE/Cy7

IL-3

BVD3-1F9

PE

 

Cell subset

Acceptance Criteria, % positive of lymphocyte gate

CD3+IL-2+

10-40

CD3+IL-3+

0.2-5

CD3+ IL-4+

0.1-3

CD3+ IL-5+

0.2-3

CD3+IL-8+

0.1-5

CD3+IL-13+

0.2-5

CD3+IL-17A+

0.1-2

CD3+IFN gamma+

5-30

CD3+TNF alpha+

10-60

CD3+GM-CSF+

1-15

Note on indicated ranges: The antibodies used for testing were the following: CD3 APC/Cy7 (clone SK7), IL-2 APC/Cy7 (clone MQ1-17H12), IL-3 PE (clone BVD3-1F9), IL-4 PE/Cy7 (clone MP4-25D2), IL-5 APC (clone TRFK5), IL-8 PE/Cy7 (clone E8N1), IL-13 APC (clone JES10-5A2), IL-17a Brilliant Violet™ 421 (clone BL168), GM-CSF Pacific Blue™ (clone BVD2-21C11), TNF-α PerCP/Cyanine5.5 (clone MAB11), and IFN-ʎ APC (clone 4S.B3). Variability in antibodies used, instruments, staining conditions and other factors may influence end-user values. The Certificate of Analysis specific values are provided as a reference. It is recommended that individual laboratories establish its own control values and ranges for each parameter based on laboratory specific conditions and protocols.

Please use our CoA Look-Up Tool to find the expected lot-specific frequencies of certain cell populations included within each lot of Veri-Cells™ Activated (Cytokine) PBMC. 

Additional Product Notes

Veri-Cells™ Buffer A Plus is an improved version of the original Veri-Cells™ Buffer A to improve staining performance with tandem dyes.

Antigen Details

Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 06/03/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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