Ultra-LEAF™ Purified Rat IgG2a, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
RTK2758 (See other available formats)
Regulatory Status
RUO
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
400543 100 µg $95.00
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400544 1 mg $135.00
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400565 5 mg $490.00
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400566 25 mg $1280.00
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400573 50 mg $2140.00
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400574 100 mg $3075.00
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Description

The RTK2758 immunoglobulin reacts with KLH. The isotype of this antibody is rat IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical data sheet

Product Details

Reactivity
KLH
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Trinitrophenol + KLH
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
ICFC, WB, IP, ICC, IHC, FA - Reported in the literature, not verified in house

Recommended Usage

Each lot of this rat IgG2a, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.

Application Notes

Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), and Functional Assay (FA).

Application References

(PubMed link indicates BioLegend citation)
  1. Nishimoto H, et al. 2005. Blood 106:4241.
  2. Seach N, et al. 2008. J. Immunol. 180:5384. PubMed
  3. Jiang P, et al. 1999. J. Biol. Chem. 274:559. (FA)
Product Citations
  1. Bacigaluppi M, et al. 2016. J Neurosci. 36: 10529 - 10544. PubMed
  2. Kumagai S, et al. 2020. Immunity. 53(1):187-203.e8. PubMed
  3. Mbofung R, et al. 2017. Nat Commun. 10.1038/s41467-017-00449-z. PubMed
  4. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  5. Walker MT, et al. 2018. J Allergy Clin Immunol. 141:1711. PubMed
  6. Kugel CH, et al. 2018. Clin Cancer Res. 24:5347. PubMed
  7. Pilch Z, et al. 2018. Cancer Immunol Res. 6:930. PubMed
  8. Weckbach LT, et al. 2019. J Exp Med. 216:350. PubMed
  9. Shimizu K, et al. 2020. Molecular Cell. 77(5):937-950.e6.. PubMed
RRID
AB_11148951 (BioLegend Cat. No. 400543)
AB_11147167 (BioLegend Cat. No. 400544)

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All IgG2a, κ Isotype Ctrl Reagents Request Custom Conjugation
Description Clone Applications
APC Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
Biotin Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
FITC Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
PE Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
PE/Cyanine5 Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
Purified Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC, WB, IP, ICC, IHC, FA, ChIP
Brilliant Violet 650™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
TotalSeq™-A0238 Rat IgG2a, κ Isotype Ctrl RTK2758 PG
Brilliant Violet 711™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
Go-ChIP-Grade™ Purified Rat IgG2a, κ Isotype Ctrl RTK2758 ChIP, FC
APC/Cyanine7 Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
Brilliant Violet 785™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
GoInVivo™ Purified Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC, WB, IP, ICC, IHC, FA
APC/Fire™ 810 Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
TotalSeq™-B0238 Rat IgG2a, κ Isotype Ctrl RTK2758 PG
KIRAVIA Blue 520™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Alexa Fluor® 488 Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
Alexa Fluor® 647 Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
Spark Violet™ 538 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
PE/Cyanine7 Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
PerCP Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
PerCP/Cyanine5.5 Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
Brilliant Violet 421™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
Alexa Fluor® 594 Rat IgG2a, κ Isotype Ctrl RTK2758 ICC
Spark Blue™ 550 Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
Brilliant Violet 570™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
Brilliant Violet 605™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
Brilliant Violet 510™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
PE/Dazzle™ 594 Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
APC/Fire™ 750 Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
TotalSeq™-C0238 Rat IgG2a, κ Isotype Ctrl RTK2758 PG
Spark YG™ 593 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Pacific Blue™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
Alexa Fluor® 700 Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
Spark YG™ 581 Rat IgG2a, κ Isotype Ctrl RTK2758 FC, ICFC
Go To Top Version: 4    Revision Date: 06/06/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC Rat IgG2a, κ Isotype Ctrl

  • Biotin Rat IgG2a, κ Isotype Ctrl

  • FITC Rat IgG2a, κ Isotype Ctrl

  • PE Rat IgG2a, κ Isotype Ctrl

  • PE/Cyanine5 Rat IgG2a, κ Isotype Ctrl

  • Purified Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 650™ Rat IgG2a, κ Isotype Ctrl

  • TotalSeq™-A0238 Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 711™ Rat IgG2a, κ Isotype Ctrl

  • Go-ChIP-Grade™ Purified Rat IgG2a, κ Isotype Ctrl

  • APC/Cyanine7 Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 785™ Rat IgG2a, κ Isotype Ctrl

  • GoInVivo™ Purified Rat IgG2a, κ Isotype Ctrl

  • APC/Fire™ 810 Rat IgG2a, κ Isotype Ctrl

  • TotalSeq™-B0238 Rat IgG2a, κ Isotype Ctrl

  • KIRAVIA Blue 520™ Rat IgG2a, κ Isotype Ctrl

  • Alexa Fluor® 488 Rat IgG2a, κ Isotype Ctrl

  • Alexa Fluor® 647 Rat IgG2a, κ Isotype Ctrl

  • Spark Violet™ 538 Rat IgG2a, κ Isotype Ctrl

  • PE/Cyanine7 Rat IgG2a, κ Isotype Ctrl

  • PerCP Rat IgG2a, κ Isotype Ctrl

  • PerCP/Cyanine5.5 Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 421™ Rat IgG2a, κ Isotype Ctrl

  • Alexa Fluor® 594 Rat IgG2a, κ Isotype Ctrl

  • Spark Blue™ 550 Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 570™ Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 605™ Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 510™ Rat IgG2a, κ Isotype Ctrl

  • PE/Dazzle™ 594 Rat IgG2a, κ Isotype Ctrl

  • APC/Fire™ 750 Rat IgG2a, κ Isotype Ctrl

  • TotalSeq™-C0238 Rat IgG2a, κ Isotype Ctrl

  • Spark YG™ 593 Rat IgG2a, κ Isotype Ctrl

  • Pacific Blue™ Rat IgG2a, κ Isotype Ctrl

  • Alexa Fluor® 700 Rat IgG2a, κ Isotype Ctrl

  • Spark YG™ 581 Rat IgG2a, κ Isotype Ctrl

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