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Ultra-LEAF™ Purified Human IgG1 Isotype Control Recombinant Antibody

Pricing & Availability
Clone
QA16A12 (See other available formats)
Regulatory Status
RUO
Other Names
IGHG1, constant region of heavy chain of IgG1, immunoglobulin gamma 1
Isotype
Human IgG1, κ
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Product Citations
publications
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Cat # Size Price Quantity Check Availability Save
403501 100 µg $248
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403502 1 mg $495
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Description

Immunoglobulin G1, or IgG1, is a type of antibody. Humans express four types of IgG (IgG1, IgG2, IgG3, and IgG4). Antibodies in mammals are complex proteins, composed of four independent amino acid chains. The molecule is formed by two identical heavy chains and two identical light chains, and produced by B cells. The FcγR molecules (CD16, CD32, and CD64) on the surface of various immune cells can bind IgG1, IgG2, and IgG4.  

Product Details
Technical data sheet

Product Details

Antibody Type
Recombinant
Host Species
Human
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Elisa Control - Verified 
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.

Application Notes

QA16A12 is a full length recombinant human IgG1 consisting of both heavy and light chains. It can be used as an isotype control when evaluating fully human, humanized, or chimeric antibodies. This product is suitable as an isotype-matched control for ELISA, immunoblotting, FACS, IHC, and functional studies.

Product Citations
  1. Roberts A, et al. 2021. Sci Rep. 11:4030. PubMed
  2. Ho CH, et al. 2021. Arthritis Res Ther. 23:199. PubMed
  3. Masuta Y, et al. 2022. iScience. 25:105085. PubMed
  4. Tahk S, et al. 2021. J Hematol Oncol. 14:155. PubMed
  5. Shiomi M, et al. 2021. Cancer Sci. 112:3655. PubMed
  6. Park HS, et al. 2022. NPJ Vaccines. 7:72. PubMed
  7. Kim A, et al. 2019. J Immunol. 203:1076. PubMed

Antigen Details

Structure
Immunoglobulin, 150 KDa
Cell Type
B cells
Biology Area
Immunology
Gene ID
NA

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 03/24/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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