Ultra-LEAF™ Purified anti-Syntrophin Antibody

Pricing & Availability
Clone
1351 (See other available formats)
Other Names
Dystrophin-associated protein A1 acidic component 1, Pro-TGF-alpha cytoplasmic domain-interacting protein 1, TACIP1, Syntrophin-1, SNT1, LQT12,dJ1187J4.5
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1351_PURE_Syntrophin_Antibody_WB_090915
Western blot showing human and mouse reactivity with clone 1351, which was tested on 15 µg/lane of HeLa, PC-3, 3T3, and HUVEC lysates and was tested at 1 µg/ml. The blot was incubated with HRP anti-mouse IgG secondary antibody and GAPDH antibody was used as a positive control.
  • 1351_PURE_Syntrophin_Antibody_WB_090915
    Western blot showing human and mouse reactivity with clone 1351, which was tested on 15 µg/lane of HeLa, PC-3, 3T3, and HUVEC lysates and was tested at 1 µg/ml. The blot was incubated with HRP anti-mouse IgG secondary antibody and GAPDH antibody was used as a positive control.
Cat # Size Price Quantity Avail. Save
845103 100 µg $325
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Description

Syntrophins are a family of five 60 kD adapter proteins that use their multiple protein interaction domains (two PHDs and a PDZ) to localize various signaling proteins (kinases, ion/water channels, or NOS) to specific intracellular locations. In neurons, syntrophins are known as the postsynaptic density protein (PSD) that serves to connect neurotransmitter receptors (such as nAChRs) to the cytoskeleton.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Whole purified syntrophin from Torpedo californica electric organ postsynaptic membrane.
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The epitope has been mapped to amino acids 108-120 within the α-syntrophin PDZ domain.

Application References

(PubMed link indicates BioLegend citation)
  1. Froehner S, et al. 1987. J. Cell Biol. 104:1633. (WB, IHC)
  2. Gee S, et al. 1998. J. Neurosci. 18:128. (IP)
  3. Ort T, et al. 2001. EMBO 20:4013. (IP, WB)
  4. Adams M, et al. 2001. J. Cell Biol. 155:113. (IF)
  5. Williams M, et al. 1999. J. Cell Biol. 144:1259. (IHC)
RRID
AB_2566134 (BioLegend Cat. No. 845103)

Antigen Details

Structure
Monomer and homodimer. Syntrophins consist of a conserved domain, two pleckstin homology domains, a PDZ domain, and a highly conserved C-terminal syntrophin unique (SU) domain. Expected MW: 58 kD.
Function
Adapter protein that binds to and probably organizes the subcellular localization of a variety of membrane proteins. May link various receptors to the actin cytoskeleton and the dystrophin glycoprotein complex.
Interaction
Interacts with the other members of the syntrophin family SNTB1 and SNTB2; SGCG and SGCA of the dystrophin glycoprotein complex; NOS1; GRB2; the sodium channel proteins SCN4A and SCN5A; F-actin and calmodulin; Interacts with MYOC.
Biology Area
Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
Postsynaptic proteins
Gene ID
6640 View all products for this Gene ID
UniProt
View information about Syntrophin on UniProt.org

Related FAQs

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 09/17/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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