Ultra-LEAF™ Purified anti-mouse CD275 (B7-H2, B7-RP1, ICOS Ligand) Antibody

Pricing & Availability
Clone
HK5.3 (See other available formats)
Regulatory Status
RUO
Other Names
ICOS ligand, ICOSL, B7 homolog 2, B7-H2, B7H2, B7RP1, B7h, ICOS-L
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
HK5dot3_PE_021808
C57Bl/6 mouse splenocytes stained with CD275 (clone HK5.3) PE
  • HK5dot3_PE_021808
    C57Bl/6 mouse splenocytes stained with CD275 (clone HK5.3) PE
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107411 100 µg $110
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107410 1 mg $149
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107412 5 mg $539
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107413 25 mg $1408
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107414 50 mg $2354
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107415 100 mg $3383
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Description

CD275 is a 40 kD protein, also known as ICOS ligand, B7-RP1, B7-H2, and B7h. It is a member of the immunoglobulin receptor superfamily expressed on splenic B cells, dendritic cells, and macrophages. The expression of this antigen is increased by TNF-α stimulation and is highly upregulated by local inflammation. B7RP1 binds to ICOS and co-stimulates T cell and B cell responses. Two isoforms of this protein have been reported to occur as a result of alternative splicing. These isoforms show differential tissue distribution.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse B7-RP1 transfected cell line
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of ligand binding1,2. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 107405) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 107404) or biotinylated anti-rat IgG second step (Cat. No. 405402), followed by SAv-PE (Cat. No. 405204)). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 107410) (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Iwai H, et al. 2003. J. Immunol. 171:2848. (Block)
  2. Kohyama M, et al. 2004. P. Natl. Acad. Sci. USA 101:4192. (Block)
  3. Zhang P, et al. 2007. J.Immunol. 178:474. PubMed
  4. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Product Citations
  1. Verma M, et al. 2021. J Exp Med. 218:. PubMed
  2. Rivera CA, et al. 2022. Immunity. 55:129. PubMed
RRID
AB_2832361 (BioLegend Cat. No. 107411)
AB_11149485 (BioLegend Cat. No. 107410)
AB_2832362 (BioLegend Cat. No. 107412)
AB_2832363 (BioLegend Cat. No. 107413)
AB_2832364 (BioLegend Cat. No. 107414)
AB_2832365 (BioLegend Cat. No. 107415)

Antigen Details

Structure
B7 Immunoglobulin superfamily, approximately 40 kD
Distribution

Isoform 1 has highest expression in lymphoid tissues, splenic B-cells, T-cells, dendritic cells and macrophages; lower levels in many non-lymphoid tissues. The expression of isoform 2 is restricted to heart, spleen, and kidney.

Function
Binds to ICOS. Co-stimulates T cell responses including proliferation and cytokine secretion. Stimulates B cell proliferation and differentiation to plasma cells.
Ligand/Receptor
ICOS
Cell Type
B cells, Dendritic cells, Lymphocytes, Macrophages, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Yoshinaga SK, et al. 1999. Nature 402:827.
2. Ling V, et al. 2000. J. Immunol. 164:1653.
3. Ling V, et al. 2001. J. Immunol. 164:1653.

Gene ID
50723 View all products for this Gene ID
UniProt
View information about CD275 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-mouse CD275 (B7-H2, B7-RP1, ICOS Ligand)

  • PE anti-mouse CD275 (B7-H2, B7-RP1, ICOS Ligand)

  • Ultra-LEAF™ Purified anti-mouse CD275 (B7-H2, B7-RP1, ICOS Ligand)

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