- HK5.3 (See other available formats)
- Regulatory Status
- Other Names
- ICOS ligand, ICOSL, B7 homolog 2, B7-H2, B7H2, B7RP1, B7h, ICOS-L
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
CD275 is a 40 kD protein, also known as ICOS ligand, B7-RP1, B7-H2, and B7h. It is a member of the immunoglobulin receptor superfamily expressed on splenic B cells, dendritic cells, and macrophages. The expression of this antigen is increased by TNF-α stimulation and is highly upregulated by local inflammation. B7RP1 binds to ICOS and co-stimulates T cell and B cell responses. Two isoforms of this protein have been reported to occur as a result of alternative splicing. These isoforms show differential tissue distribution.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Mouse B7-RP1 transfected cell line
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
FC - Quality tested
Block - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of ligand binding1,2. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 107405) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 107404) or biotinylated anti-rat IgG second step (Cat. No. 405402), followed by SAv-PE (Cat. No. 405204)). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 107410) (Endotoxin <0.01 EU/µg).
(PubMed link indicates BioLegend citation)
- Product Citations
AB_2832361 (BioLegend Cat. No. 107411)
AB_11149485 (BioLegend Cat. No. 107410)
AB_2832362 (BioLegend Cat. No. 107412)
AB_2832363 (BioLegend Cat. No. 107413)
AB_2832364 (BioLegend Cat. No. 107414)
AB_2832365 (BioLegend Cat. No. 107415)
- B7 Immunoglobulin superfamily, approximately 40 kD
Isoform 1 has highest expression in lymphoid tissues, splenic B-cells, T-cells, dendritic cells and macrophages; lower levels in many non-lymphoid tissues. The expression of isoform 2 is restricted to heart, spleen, and kidney.
- Binds to ICOS. Co-stimulates T cell responses including proliferation and cytokine secretion. Stimulates B cell proliferation and differentiation to plasma cells.
- Cell Type
- B cells, Dendritic cells, Lymphocytes, Macrophages, T cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
1. Yoshinaga SK, et al. 1999. Nature 402:827.
2. Ling V, et al. 2000. J. Immunol. 164:1653.
3. Ling V, et al. 2001. J. Immunol. 164:1653.
- Gene ID
- 50723 View all products for this Gene ID
- View information about CD275 on UniProt.org
- Do you guarantee that your antibodies are totally pathogen free?
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
|Biotin anti-mouse CD275 (B7-H2, B7-RP1, ICOS Ligand)||HK5.3||FC|
|PE anti-mouse CD275 (B7-H2, B7-RP1, ICOS Ligand)||HK5.3||FC|
|Ultra-LEAF™ Purified anti-mouse CD275 (B7-H2, B7-RP1, ICOS Ligand)||HK5.3||FC, Block|
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