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Ultra-LEAF™ Purified anti-mouse CD253 (TRAIL) Antibody

Pricing & Availability
Clone
N2B2 (See other available formats)
Regulatory Status
RUO
Other Names
TNFSF10, TNF-related apoptosis inducing ligand, Apo-2 ligand, Apo-2L, CD253
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
N2B2_LEAF_091907.jpg
Mouse TRAIL transfected cells stained with LEAF™ purified N2B2, followed by anti-rat IgG FITC
  • N2B2_LEAF_091907.jpg
    Mouse TRAIL transfected cells stained with LEAF™ purified N2B2, followed by anti-rat IgG FITC
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109315 100 µg $237
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109316 1 mg $688
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Description

CD253 is a 40 kD TNF superfamily member known as TRAIL, Apo-2 ligand, and Apo-2L. TRAIL is expressed on a variety of cells, including IL-2 and IL-15 activated NK cells and activated T cells. However, it is undetectable on resting T and B cells. TRAIL has been reported to induce apoptosis in tumor and transformed cell lines by a caspase-dependent process. The N2B2 antibody has been reported to be useful for flow cytometric staining and blocking NK cell cytotoxicity in vitro.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse TRAIL-transfected 2PK-3 cells
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Qualit tested
Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of NK cell cytotoxicity1,2. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109308).

Application References

(PubMed link indicates BioLegend citation)
  1. Kayagaki N, et al. 1999. J. Immunol. 163:1906. (Block)
  2. Sato K, et al. 2005. J. Immunol. 174:4025. (Block)
  3. Joshi PS, et al. 2006. J. Leukocyte Biol. 80:1345.
  4. Herold S, et al. 2008. J. Exp. Med. 205:3065. PubMed
  5. Iannello A, et al. 2009. J. Virol. 83:5999. PubMed
  6. Komatsu M, et al. 2003. Blood 101:3991. (Block)
  7. Taieb J, et al. 2006. Nature Med. 12:214. (Block)
  8. Gonzaga R, et al. 2011. J. Immunol. 187:6235. PubMed.
RRID
AB_2813976 (BioLegend Cat. No. 109315)
AB_2813977 (BioLegend Cat. No. 109316)

Antigen Details

Structure
TNF ligand superfamily member, approximately 40 kD
Distribution

Widespread, highest expression in spleen, lung, prostate; NK, monocytes, neutrophils, activated T cells

Function
Induces apoptosis in tumorigenic and transformed cell lines, involved in T cell mediated cytotoxicity
Ligand/Receptor
TRAIL receptor DR5
Cell Type
Monocytes, Neutrophils, NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Kayagaki N, et al. 1999. J. Immunol. 163:1906.
2. Wiley SR, et al. 1995. Immunity. 3:673.
3. Wu GS, et al. 1999. Cancer Res. 59:2770.
4. Mariani SM, et al. 1998. Eur. J. Immunol. 28:973.

Gene ID
22035 View all products for this Gene ID
UniProt
View information about CD253 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 08/15/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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