Ultra-LEAF™ Purified anti-mouse CD182 (CXCR2) Antibody

Pricing & Availability
Clone
SA045E1 (See other available formats)
Other Names
IL-8RB, GRO/MGSA, Cmkar2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
SA045E1_ULEAF_CD182_Antibody_FC_1_111915
C57BL/6 mouse bone marrow cells were stained with Ultra-LEAF™ purified CXCR2 (clone SA045E1, top) or Ultra-LEAF™ purified rat IgG2a, κ isotype control (bottom) followed by anti-rat IgG PE and Ly-6C/Ly-6G APC.
  • SA045E1_ULEAF_CD182_Antibody_FC_1_111915
    C57BL/6 mouse bone marrow cells were stained with Ultra-LEAF™ purified CXCR2 (clone SA045E1, top) or Ultra-LEAF™ purified rat IgG2a, κ isotype control (bottom) followed by anti-rat IgG PE and Ly-6C/Ly-6G APC.
  • SA045E1_ULEAF_CD182_Antibody_FC_2_111915
Cat # Size Price Quantity Avail. Save
149611 100 µg $265
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Description

CD182, also known as CXCR2, is a G-protein coupled receptor with seven transmembrane regions that is involved in chemotaxis, neutrophil activation, and angiogenesis. CXCR2 is expressed by neutrophils, basophils, a subset of T cells, monocytes, macrophages, natural killer (NK) and natural killer T cells (NKT). Its ligands are CXCL1, CXCL2, CXCL3, and CXCL5.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CXCR2-transfected cells.
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 149611) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01EU/µg).

RRID
AB_2566149 (BioLegend Cat. No. 149611)

Antigen Details

Structure
G-protein coupled receptor and seven transmembrane regions.
Distribution
Neutrophils, basophils, subset of T cells, monocytes, macrophages, and natural killer (NK) cells.
Function
Chemotaxis, neutrophil activation, and angiogenesis.
Ligand/Receptor
CXCL1, CXCL2, CXCL3, and CXCL5.
Cell Type
Neutrophils, Basophils, T cells, Monocytes, Macrophages, NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Spaan AN, et al. 2014. Nat. Commun. 5:5438.
2. Natsuaki Y, et al. 2014. Nat. Immunol. 15:1064.
3. Hsieh CY, et al. 2014. J. Immunol. 193:3693.
4. Diana J and Lehuen A. 2014. EMBO Mol. Med. 6:1090.
5. Stoolman JS, et al. 2014. J. Immunol. 193:564.

Gene ID
12765 View all products for this Gene ID
UniProt
View information about CD182 on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 11/23/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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