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Ultra-LEAF™ Purified anti-human/mouse integrin β7 Antibody

Pricing & Availability
Clone
FIB27 (See other available formats)
Regulatory Status
RUO
Other Names
β7 Integrin, integrin βp, ITGB7
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
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121011 100 µg $270
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121012 1 mg $743
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Description

Integrin β7 is a 130 kD glycoprotein, also known as integin βp. It is a member of the Ig superfamily. In association with integrin α4 or αE chain, β7 forms α4/β7 or αE/β7 heterodimer. α4/β7 (CD49d/β7, LPAM-1) is expressed on majority of peripheral lymphocytes, small subsets of thymocytes and bone marrow progenitors. LPAM-1 binds to several ligands, VCAM-1, MAdCAM-1 and fibronectin, and is involved in lymphocyte adhesion, some hematopoietic progenitor cells migration. αE/β7 (CD103/β7,αIEL/β7) is expressed on intestinal intraepithelial lymphocytes (IEL), dendritic epidermal T cells, T regulatory cells, a subset of CD8+ T cells in lymph nodes and lamina propria. CD103/β7 complex is thought to play a role in lymphocyte retention via interaction with its lignd E-Cadherin. The FIB27 antibody has been reported to react with mouse and human β7 integrin and to block β7 integrin-mediated cell adhesion in in vitro and in vivo studies.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TK1 cells
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
IP, Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block)
  2. Berlin C, et al. 1993. Cell 74:185. (Block)
  3. Tidswell M, et al. 1997. J. Immunol. 159:1497. (Block)
RRID
AB_2832429 (BioLegend Cat. No. 121011)
AB_2832430 (BioLegend Cat. No. 121012)

Antigen Details

Structure
Integrin family, Ig superfamily, 130 kD, heterodimer with CD49d or CD103
Distribution

CD49d/ß7: majority of lymphocytes, thymocyte subset, and some bone marrow progenitors; CD103/ß7: intestinal intraepithelial lymphocytes (IEL), dendritic epidermal T cells, subset of CD8 T cells in lymph nodes

Function
Lymphocyte adhesion, hematopoietic progenitor cells migration
Ligand/Receptor
CD49d/ß7: VCAM-1 (CD106), MAdCAM-1 and fibronectin; CD103/ß7: E-cadherin
Cell Type
Hematopoietic stem and progenitors, Lymphocytes, T cells, Thymocytes, Tregs
Biology Area
Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Andrew DP, et al. 1994. J. Immunol. 153:3847.
2. Picarella D, et al. 1997. J. Immunol. 158:2099.
3. Lefrancois L, et al. 1994. Eur. J. Immunol. 24:635
4. Cepek KL, et al. 1994. Nature 372:190.

Gene ID
16421 View all products for this Gene ID 3695 View all products for this Gene ID
UniProt
View information about Integrin beta7 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 03/09/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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