Ultra-LEAF™ Purified anti-human IL-3 Antibody

Pricing & Availability
BVD8-3G11 (See other available formats)
Regulatory Status
Other Names
Interleukin-3, Burst promoting activity, Eosinophil colony stimulating factor (Eo-CSF), Hematopoietic cell growth factor (HCGF), Mast (MGF/MCGF), Multi-colony stimulating (Multi-CSF), P cell stimulating activity (PCSA), Thy1 inducing factor
Rat IgG1, κ
Ave. Rating
Submit a Review
Product Citations
Ultra-LEAF™ Purified anti-human IL-3 antibody inhibits the proliferation of TF-1 erythroleukemic cells induced by Recombinant Human IL-3 (carrier-free) (7 ng/ml) in a dose dependent manner, with an ED50 = 0.02 – 0.1 µg/ml.
  • BVD8dash3G11_ULEAF_IL3_Antibody_Block_041916
    Ultra-LEAF™ Purified anti-human IL-3 antibody inhibits the proliferation of TF-1 erythroleukemic cells induced by Recombinant Human IL-3 (carrier-free) (7 ng/ml) in a dose dependent manner, with an ED50 = 0.02 – 0.1 µg/ml.
Cat # Size Price Quantity Check Availability Save
500506 500 µg $325
Check Availability

Need larger quantities of this item?
Request Bulk Quote

IL-3 is a highly species-specific pleiotropic factor produced primarily by activated T cells though also by mast cells, keratinocytes, and astrocytes, which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. The BVD8-3G11 antibody can neutralize the bioactivity of natural or recombinant IL-3.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Yeast-expressed, recombinant human IL-3
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

Block - Quality tested
IP, WB - Reported in the literature, not verified in house

Recommended Usage

Ultra-LEAF™ Purified anti-human IL-3 antibody inhibits the proliferation of TF-1 erythroleukemic cells induced by Recombinant Human IL-3 (carrier-free) (7 ng/ml) in a dose dependent manner, with an ED50 = 0.02 – 0.1 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA1-4 or ELISPOT Capture: The purified BVD8-3G11 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated BVD3-1F9 antibody (Cat. No. 500604) as the detecting antibody.
Additional reported applications include: Western blotting4 and immunoprecipitation4.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams JS, et al. 1992. Immunol. Rev. 127:5. (ELISA)
  2. Abrams JS et al. 1994. Eosnophil-active cytokines in human disease: development and use of monoclonal antibodies to il-3, il-5, and GM-CSF. In Eosinophils in Allergy and Inflammation. Gleich GJ and Kay AB, eds. Marcel Dekker, Inc. New York, NY. p. 133. (ELISA)
  3. Abrams J. 1995. Curr. Prot. Immunol. 6.20. (ELISA)
  4. Kaushansky K. 1992. J. Clin. Invest. 90:1879. (ELISA, IP, WB)
  5. Mahanty S, et al. 1992. J. Immunol. 148:3567.
AB_2616770 (BioLegend Cat. No. 500506)

Antigen Details

Cytokine; 14-30 kD (Mammalian)
Proliferation/differentiation of almost all types of hematopoietic progenitors into granulocytes, macrophages, erythroid cells, megakaryocytes, mast cell colonies; induces expression of 20-α-steroid dehydrogenase, histidine, ornithine decarboxylase.
Cell Sources
Activated T cells, mast cells, eosinophils, keratinocytes, NK cells, endothelial cells.
Cell Targets
Erythroid cells, megakaryocytes, neutrophils, eosinophils, basophils, mast cells, monocytic lineages.
IL-3R, α subunit (CD123), common β subunit (CDw131).
Biology Area
Cell Biology, Stem Cells
Molecular Family
Growth Factors, Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Frendl G. 1992. Int. J. Immunopharmacol. 14:421.
3. Ihle J. 1992. Chem. Immunology 51:65.

Upregulated by T cell stimulation; downregulated by glucocorticoids.
Gene ID
3562 View all products for this Gene ID
View information about IL-3 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All IL-3 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human IL-3 BVD8-3G11 ELISA Capture, IP, WB
Ultra-LEAF™ Purified anti-human IL-3 BVD8-3G11 Block, IP, WB
Go To Top Version: 1    Revision Date: 04/20/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human IL-3

  • Ultra-LEAF™ Purified anti-human IL-3


Remember me
Forgot your password? Reset Password
Request an Account