Ultra-LEAF™ Purified anti-human CD41/CD61 Antibody

Pricing & Availability
A2A9/6 (See other available formats)
Regulatory Status
Other Names
Mouse IgG2a, κ
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Product Citations
Thrombin-activated human peripheral blood platelets were stained with mouse IgG1 anti-CD62P APC and purified CD41/CD61 (clone A2A9/6) (top) or purified mouse IgG2a, κ isotype control (bottom), followed by anti-mouse IgG2a PE.
  • A2A9_Purified_CD41CD61_Antibody_FC_1_082913.jpg
    Thrombin-activated human peripheral blood platelets were stained with mouse IgG1 anti-CD62P APC and purified CD41/CD61 (clone A2A9/6) (top) or purified mouse IgG2a, κ isotype control (bottom), followed by anti-mouse IgG2a PE.
  • A2A9_Purified_CD41CD61_Antibody_FC_2_082913.jpg
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359804 500 µg $325
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CD41/CD61, also known as gpIIb/IIIa, is a member of a family integrin receptors. This is a complex comprised by CD41 and CD61 through non-covalent association. CD41/CD61 is mainly expressed by platelets and megakaryocytes. The resting form of the CD41/CD61 complex is involved in platelet activation and aggregation by binding to immobilized fibrinogen. After activation, CD41/CD61 becomes a receptor for soluble fibrinogen and several other RGD-containing adhesive proteins such as von Willebrand Factor (vWF) and fibronectin. An absence or dysfunction of CD41/CD61 on the platelet surface results in an inherited bleeding disorder, called Glanzmann Thromsasthenia (GT). CD41/CD61 has been found on murine hematopoietic progenitor cells, indicating that this complex may play a role in regulating hematopoietic development.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Human platelets
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

FC - Quality tested
Block, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for relevant formats) include: immunoprecipitation1 and blocking (aggregation)2. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 359804).

A2A9/6 is recognized as having high affinity to gpIIb/IIIa. It has been shown to inhibit platelet aggregation induced by a variety of agonists and the initiation of clot formation.

Application References

(PubMed link indicates BioLegend citation)
  1. Basani RB, et al. 1996. Blood. 88:167. (IP)
  2. Bennett JS, et al. 1983. Proc. Natl. Acad. Sci. USA. 80:2417. (Block)
Product Citations
  1. Zheng L, et al. 2021. Front Cell Infect Microbiol. 11:783085. PubMed
  2. Posner M, et al. 2016. J Biol Chem. 291: 2764 - 2776.. PubMed
AB_2562484 (BioLegend Cat. No. 359804)

Antigen Details

Complex of CD41 and CD61 through non-covalent association.
Platelets and megakaryocytes.
Mediates attachment to cells of diverse matrix proteins.
Fibrinogen, RGD-containing adhesive proteins such as vonWillebrand Factor (vWF) and fibronectin.
Cell Type
Platelets, Megakaryocytes
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Matsumura-Takeda K, et al. 2007. Stem Cell. 25:862.
2. Corbel C, et al. 2005. 49:279.
3. Bennett JS, et al. 1983. Proc. Natl. Acad. Sci. USA. 80:2417. 
4. Clemetson KJ, et al. 1994. Curr. Opin. Hematol. 1:388.

Gene ID
3690 View all products for this Gene ID 3674 View all products for this Gene ID
View information about CD41/CD61 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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