Ultra-LEAF™ Purified anti-human CD307c/FcRL3 Antibody

Pricing & Availability
Clone
H5/FcRL3 (See other available formats)
Other Names
Fc receptor-like 3 (FCRL3), Immune receptor translocation-associated protein 3 (IRTA3), SH2 domain-containing phosphatase anchor protein 2 (SPAP2), IFGP family protein 3 (IFGP3)
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
H5slashFcRL3_ULEAF_Purified_CD307c_Antibody_1_082417
Human peripheral blood lymphocytes were surface stained with CD3 Alexa Fluor® 488 and Ultra-LEAF™ Purified CD307c/FcRL3 (clone H5/FcRL3) antibody (top) or Mouse IgG2b, κ Ultra-LEAF™ Purified isotype control (bottom), followed by anti-mouse IgG2b PE antibody.
  • H5slashFcRL3_ULEAF_Purified_CD307c_Antibody_1_082417
    Human peripheral blood lymphocytes were surface stained with CD3 Alexa Fluor® 488 and Ultra-LEAF™ Purified CD307c/FcRL3 (clone H5/FcRL3) antibody (top) or Mouse IgG2b, κ Ultra-LEAF™ Purified isotype control (bottom), followed by anti-mouse IgG2b PE antibody.
  • H5slashFcRL3_ULEAF_Purified_CD307c_Antibody_2_082417
Cat # Size Price Quantity Avail. Save
374403 100 µg $225
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374404 1 mg $650
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Description

CD307c is a type 1 transmembrane glycoprotein in the FcRL family of the Ig gene superfamily that is conserved in humans and not found in mice. It is expressed on NK cells, T cell, regulatory T cell, B cell and plasma cell subsets. Intracellular ITAM and ITIM motifs in conjunction with extracellular Ig domains are thought to play a role in the regulation of immune response, and autoimmune function. Impairment of normal FcRL3 function has been linked to systemic lupus erythematosus, rheumatoid arthritis, and autoimmune thyroid disease.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
FcRL3 full length expression plasmid DNA, followed by cell boost using transiently transfected cells with the same plasmid.
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 μg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Nagata S, et al. 2009. J. Immunol. 182:7518-26.
RRID
AB_2715834 (BioLegend Cat. No. 374403)
AB_2715835 (BioLegend Cat. No. 374404)

Antigen Details

Distribution

NK, T, regulatory T, B, and plasma cell subsets.

Interaction
Tyrosine-protein phosphatase non-receptor type 6 (PTPN6).
Cell Type
B cells, NK cells, Plasma cells, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Bin Dhuban K, et al. 2015. J. Immunol. 194(8):3687-96.
2. Yuan M, et al. 2016. Mol. Neurobiol. 53:2029-35.
3. Fang Y, et al. 2016. Immunobiology. 221(1):56-62.

Gene ID
115352 View all products for this Gene ID
UniProt
View information about CD307c/FcRL3 on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 09/01/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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