Ultra-LEAF™ Purified anti-human CD284 (TLR4) Antibody

Pricing & Availability
Clone
HTA125 (See other available formats)
Workshop
HCDM listed
Other Names
Toll-like Receptor 4, TLR4, TLR-4, CD284
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
HTA125_PE_040810
Human peripheral blood monocytes stained with HTA125 PE.
  • HTA125_PE_040810
    Human peripheral blood monocytes stained with HTA125 PE.
Cat # Size Price Quantity Avail. Save
312814 1 mg $650
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Description

Toll-like receptors are type I transmembrane signaling receptors. They are primordial pathogen-recognition proteins that function as sentinels for the innate immune system. TLR4, also known as CD284, is a 110 kD protein which is expressed on monocytes/macrophages, endothelial cells, and at low levels on B cells and granulocytes. In association with a secretory molecule, MD2, TLR4 has been recognized as critical for host recognition of bacterial LPS. HTA125 antibody is useful for flow cytometric analysis and is able to block LPS-induced cytokine production.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Cross-Reactivity: Guinea Pig
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Ba/F3 cell line expressing human TLR4
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Block, IHC, IF, WB - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood.  It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4, immunofluorescence microscopy6, Western blotting10, and in vitro blocking of LPS-induced cytokine production2,3,7,9. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 312806) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 312804) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204). The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 312807). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 312814) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Skimazu R, et al. 1999. J. Exp. Med. 189:1777.
  2. Wang R, et al. 2003. Hybrid Hybridomics 22:357. (Block)
  3. Wang JE, et al. 2001. Infect. Immun. 69:2402. (Block)
  4. Ishihara S, et al. 2004 J. Immunol. 173:1406. (IHC)
  5. Kawahara T, et al. 2001 Infect. Immun. 69:4382.
  6. Jiang Q, et al. 2000. J. Immunol. 165:3541. (IF)
  7. Sugawara S, et al. 2001. Infect. Immun. 69:4951. (Block)
  8. Chavakis E, et al. 2007. Circ. Res. 100:204. PubMed
  9. Bhattacharyya S, et al. 2007. Am. J. Physiol. Gastrointest Liver Physiol. doi:10.1152/ajpgi.00149. (Block) PubMed
  10. Baumgarten G, et al. 2001. J. Infectious. Dis. 183:1617.
RRID
AB_2561485 (BioLegend Cat. No. 312814)

Antigen Details

Structure
Type I transmembrane protein, Toll-like receptor family/IL-1R superfamily, 110 kD
Distribution

Monocytes/macrophages, endothelial cells, B cells and granulocytes

Function
Activates the innate immune system against Gram-negative bacterial pathogens by recognition of LPS
Ligand Receptor
Bacterial LPS
Cell Type
Monocytes, Macrophages, Endothelial cells, B cells, Granulocytes, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Toll Like Receptors
Antigen References

1. Skimazu R, et al. 1999. J. Exp. Med. 189:1777.

Gene ID
7099 View all products for this Gene ID
UniProt
View information about CD284 on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 4    Revision Date: 11/11/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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