Ultra-LEAF™ Purified anti-Asialo-GM1 Antibody

Pricing & Availability
Poly21460 (See other available formats)
Regulatory Status
Other Names
Ganglioside, GM1, ganglio-N-tetraosylceramide
Rabbit Polyclonal IgG
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Product Citations
Poly21460_Asialo-GM1 _Antibody_FC_062413
C57BL/6 mouse splenocytes were stained with Ultra-LEAF™ purified anti-Asialo-GM1 (clone Poly21460), followed by anti-rabbit IgG FITC and NK1.1 PE/Cy7.
  • Poly21460_Asialo-GM1 _Antibody_FC_062413
    C57BL/6 mouse splenocytes were stained with Ultra-LEAF™ purified anti-Asialo-GM1 (clone Poly21460), followed by anti-rabbit IgG FITC and NK1.1 PE/Cy7.
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146002 1 ml (reconstituted) $534
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GM1 is a ganglioside, a type of glycosphingolipid with a single sialic acid group. Asialo-GM1 is a GM1 derivative without a sialic acid group. It is expressed on NK cells, basophils, monocytes/macrophages, and T cells. It is particularly expressed on very early thymocytes, but the expression decreases as the cells mature and become Thy-1+. The highest expression is detected on neuronal tissues. This molecule has been shown to be involved in microbial pathogenesis. Antibodies specific for Asialo-GM1 are elevated in dementia, lupus, and Guillain-Barré syndrome.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Host Species
Lyophilized powder. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Reconstitute with 1 ml sterile deionized, distilled, endotoxin-free water. Gamma globulin fraction of serum was precipitated using 50% ammonium sulfate followed by dialysis with phosphate buffered saline.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

FC - Quality tested
Depletion - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Optimal staining for flow cytometry may vary from lot-to-lot. This antibody has been reported to deplete NK cells in vivo. A usage of 50 µl per mouse is recommended, but optimal usage will vary depending on the strain of mouse and experimental conditions2-4. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody recognizes asialo-GM1. It does not react with other glycolipids, such as GM1 and Asialo-GM2.

This antibody can partially block IL-12 induced IFN-γ production but does not affect other systemic action of IL-12. 

This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Naiki M, et al. 1974. J. Immunol. 113:84.
  2. Kasai M, et al. 1981. Nature. 291:334. (Depletion)
  3. Nishikado H, et al. 2011. J. Immunol. 186:5766. (Depletion)
  4. Zhou G, et al. 2013. Eur. J. Immunol. 43:929. (Depletion)
Product Citations
  1. Wang X, et al. 2022. Viruses. 14:. PubMed
  2. Hu J, et al. 2015. J Virol. 89: 10383 - 10398. PubMed
  3. Böttcher JP, et al. 2018. Cell. 172:1022. PubMed
  4. Li P, et al. 2020. Nat Commun. 3.504861111. PubMed
  5. André P et al. 2018. Cell. 175(7):1731-1743 . PubMed
  6. Lin Q, et al. 2021. Nat Commun. 12:2537. PubMed
  7. Shannon JP, et al. 2021. Immunity. 54(2):276-290.e5. PubMed
  8. Ren Y, et al. 2015. Sci Rep. 5: 18099. PubMed
  9. Turaj AH et al. 2017. Cancer cell. 32(6):777-791 . PubMed
  10. Dai HS et al. 2017. Immunity. 47(1):159-170 . PubMed
  11. Pelly VS, et al. 2021. Cancer Discov. 11:2602. PubMed
  12. Gauthier L, et al. 2019. Cell. 177:1701. PubMed
  13. Ma Q, et al. 2021. Precis Clin Med. 4:155. PubMed
  14. Bonavita E, et al. 2020. Immunity. 1215:53. PubMed
  15. Dubrot J, et al. 2021. Immunity. 54(3):571-585.e6. PubMed
  16. Manangeeswaran M, et al. 2020. Front Immunol. 11:237. PubMed
AB_2562206 (BioLegend Cat. No. 146002)

Antigen Details

Asialo-GM1 is a GM1 derivative without a sialic acid group, with the structure: βDGalp(1-3)βDGalpNAc(1-4)βDGalp(1-4)βDGlcp(1-1)Cer

NK cells, basophils, splenic T lymphocytes, highest concentrations on neurons

Supports cellular identification, neuronal plasticity, and repair mechanisms
Facilitates cellular recognition and cell-to-cell communication
Ligand for cholera toxin
Cell Type
Basophils, Neurons, NK cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References

1. Stein-Douglas K, et al. 1979. J. Exp. Med. 143:822. 
2. Kasai M, et al. 1980. Eur. J. Immunol. 10:175. 
3. Young WW Jr, et al. 1980. J. Immunol. 124:199. 
4. Bansal AS, et al. 1994. J. Clin. Path. 47:300.

Gene ID
View information about Asialo-GM1 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Ultra-LEAF™ Purified anti-Asialo-GM1

  • Alexa Fluor® 647 anti-Asialo-GM1

  • FITC anti-Asialo-GM1

  • PerCP/Cyanine5.5 anti-Asialo-GM1

  • PE anti-Asialo-GM1


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