Ultra-LEAF™ Purified anti-mouse IL-12/IL-23 p40 (monomer, dimer, heterodimer) Antibody

Pricing & Availability
Clone
C17.8 (See other available formats)
Other Names
Interleukin-12 p40, Interleukin-23 p40, Cytotoxic lymphocyte maturation factor (CLMF), Natural killer cell stimulatory factor (NKSF), CTL maturation factor (TcMF), T-cell stimulating factor (TSF)
Isotype
Rat IgG2a, κ
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
505307 100 µg $100
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505308 1 mg $135
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505309 5 mg $490
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505310 25 mg $1,280
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505311 50 mg $2,140
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505312 100 mg $3,075
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Description

The C17.8 antibody reacts with mouse IL-12 p40 subunit of the IL-12 p70 heterodimer and IL-23 p40 subunit of the IL-23 p19/p40, as well as p40 monomer and homodimer. The C17.8 antibody can neutralize the bioactivity of natural or recombinant IL-12.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, IL-12/IL-23 p40 subunit (monomer, homodimer and heterodimer IL-12 p35/p40 or IL-23 p19/p40)
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CHO expressed, recombinant mouse IL-12 p70
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
For 5 mg sizes and larger, the antibody is bottled at the concentration indicated on the vial, typically between 1 mg/ml and 3 mg/ml. For smaller sizes the concentration is 1.0 mg/ml.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

ELISA - Quality tested
Neut, ICFC, IP, WB - Reported in the literature

 

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection1,2,6-9 or ELISPOT Detection: The biotinylated C17.8 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified C15.6 antibody (Cat. No. 505202/505208) as capture antibody and recombinant mouse IL-12/IL-23 p40 (Cat. No. 576909) or recombinant mouse IL-12 p70 (Cat. No. 577009) as the standard.
Neutralization1,2,4,5,6,10: The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of mouse IL-12 bioactivity in vivo and in vitro (Cat. No. 505304). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 505308) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Additional reported applications (for relevant formats of this clone) include: Western blotting3, immunoprecipitation3, and intracellular immunofluorescent staining and flow cytometric analysis.
Note: For testing mouse IL-12 p40 (monomer, dimer, heterodimer) in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 431601 to 431606) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Kitagaki K, et al. 2002. Clin. Diagn. Lab Immunol. 9:1260.
  2. Reichmann G, et al. 1999. J. Immunol. 163:3354.
  3. Wysocka M, et al. 1995. Eur. J. Immunol. 25:672.
  4. Neucath M, et al. 1995. J. Exp. Med. 182:1281.
  5. Cousens LP, et al. 1999 J. Exp. Med. 189:1315.
  6. Gately M. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.16.
  7. O'Connell PJ, et al. 2006. Blood 107:1010.
  8. Dzhagalov I, et al. 2007. J. Immunol. 178:2113.
  9. Xu G, et al. 2007. J. Immunol. 179:5358. PubMed
  10. Sugimoto K, et al. 2007. Gastroenterology 133:124. PubMed
RRID
AB_11150770 (BioLegend Cat. No. 505307)
AB_11148948 (BioLegend Cat. No. 505308)
AB_2783330 (BioLegend Cat. No. 505309)
AB_2783331 (BioLegend Cat. No. 505310)
AB_2783332 (BioLegend Cat. No. 505311)
AB_2783333 (BioLegend Cat. No. 505312)

Antigen Details

Structure
Cytokine; monomer, heterodimer (p40:p35 or p40:p19) or homodimer (p40:p40)
Bioactivity
IL-12 p70 (p40:p35) induces IFN-γ, TNF-a production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes. IL-23 (p40:p19) induces proliferation and production of IFN-γ by human me
Cell Sources
Dendritic cells, monocytes/macrophages, B cells, T cells
Cell Targets
T cells, NK cells
Receptors
IL-12Rβ1 binds p40; dimeric with IL-12Rβ2 binds p35
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Quesniaux V. 1992. Research Immunol. 143:385.
3. Trinchieri G, et al. 1992 Prog. Growth Factor Res. 4:355.
4. Trinchieri G, et al. 1993 Immunol. Today. 14:335.
5. Oppmann B, et al. 2000 Immunity. 13:715.
6. Aggarwal S, et al. 2003 J. Biol. Chem. 278:1910.
7. Parham C, et al. 2002 J. Immunol. 168:5699.
8. Belladonna ML, et al. 2002 J. Immunol. 168:5448.
9. Lankford CS, et al. 2003 J. Leukoc. Biol. 73:49.

Regulation
Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function; p40 subunit in common with IL-23
Gene ID
16160 View all products for this Gene ID
UniProt
View information about IL-12 p40 on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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