- MHM-88 (See other available formats)
- Regulatory Status
- Other Names
- Immunoglobulin M
- Mouse IgG1, κ
- Barcode Sequence
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- Product Citations
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IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on immature and mature B cells, while IgM heavy (μ) chain is expressed intracellularly in pre-B cells.Product Details
- Verified Reactivity
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Host Species
- Human Ig cocktail
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA
- The antibody was purified by chromatography and conjugated with TotalSeq™-D oligomer under optimal conditions.
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
PG - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-D antibodies are compatible with Mission Bio’s Tapestri Single-Cell Sequencing Platform for simultaneous detection of DNA and Protein.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
- Application Notes
MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.
Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.
- Additional Product Notes
TotalSeq™-D reagents are designed to profile protein expression at single cell level. The Mission Bio Tapestri platform and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq/single-cell-dna
The barcode flanking sequences are CGAGATGACTACGCTACTCATGG (PCR handle), and GAGCCGATCTAGTATCTCAGT*C*G (capture sequence). * indicates a phosphorothioated bond, to prevent nuclease degradation.
View more applications data for this product in our Application Technical Notes.
(PubMed link indicates BioLegend citation)
- Perez-Shiyama C, et al. 2014. J Immunol. 192:5192. PubMed
AB_2922547 (BioLegend Cat. No. 314553)
- Ig family
- B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
- Cell Type
- B cells
- Biology Area
- Gene ID
- 3507 View all products for this Gene ID
- View information about IgM on UniProt.org
Related Pages & Pathways
Other FormatsView All IgM Reagents Request Custom Conjugation
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human IgM
PE anti-human IgM
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FITC anti-human IgM
APC anti-human IgM
PerCP/Cyanine5.5 anti-human IgM
Pacific Blue™ anti-human IgM
Brilliant Violet 421™ anti-human IgM
APC/Cyanine7 anti-human IgM
Brilliant Violet 570™ anti-human IgM
Brilliant Violet 510™ anti-human IgM
Brilliant Violet 605™ anti-human IgM
Brilliant Violet 650™ anti-human IgM
Purified anti-human IgM (Maxpar® Ready)
PE/Dazzle™ 594 anti-human IgM
PE/Cyanine7 anti-human IgM
Alexa Fluor® 488 anti-human IgM
Alexa Fluor® 647 anti-human IgM
Alexa Fluor® 700 anti-human IgM
Brilliant Violet 711™ anti-human IgM
TotalSeq™-A0136 anti-human IgM
TotalSeq™-C0136 anti-human IgM
Brilliant Violet 785™ anti-human IgM
APC/Fire™ 750 anti-human IgM
TotalSeq™-B0136 anti-human IgM
Spark Violet™ 423 anti-human IgM Antibody
TotalSeq™-D0136 anti-human IgM
Spark Blue™ 550 anti-human IgM