Recombinant Rat IFN-γ (ELISA Std.)

Pricing & Availability
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
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IFNg_Rat_ELISA_011614
  • IFNg_Rat_ELISA_011614
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580009 4 pack $80
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Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. 

Product Details
Technical data sheet

Product Details

Reactivity
Rat
Source
Rat IFN-γ, amino acids Gln23-Cys156 (Accession# NM_138880) was expressed in E. Coli.
Molecular Mass
16 kD
Purity
>98%
Formulation
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Storage & Handling
Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Application

ELISA - Quality tested

Recommended Usage

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of two-fold dilutions from 2000 pg/ml to 31 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This IFN-γ protein is useful as a standard for a rat IFN-γ sandwich ELISA, using unlabeled DB-1 antibody (Cat. No. 507801 or 507808) for capture and biotinylated Poly5188 antibody (Cat. No. 518803) for detection.

Antigen Details

Structure
Cytokine, dimer, 40-80 kD (Mammalian)
Distribution

CD8+ and CD4+ T cells, NK cells

Function
Upregulated by IL-2, bFGF, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
Interaction
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Ligand/Receptor
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Gene ID
25712 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 02/29/2016

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