Recombinant Rat CXCL12/SDF-1α (carrier-free)

Pricing & Availability
Other Names
Stromal Cell-Derived Factor 1, SDF-1, SDF1, C-X-C Motif Chemokine 12, C-X-C Motif Chemokine Ligand 12, SCYB12
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Product Citations
publications
Rat_CXCL12_SDF-1a_CF_RECOM_1_091118_updated
Rat CXCL12/SDF-1α chemoattracts Ba/F3-hCXCR4 transfectants in a dose dependent manner. The ED50 for this effect is 0.3 - 1.5 ng/mL.
  • Rat_CXCL12_SDF-1a_CF_RECOM_1_091118_updated
    Rat CXCL12/SDF-1α chemoattracts Ba/F3-hCXCR4 transfectants in a dose dependent manner. The ED50 for this effect is 0.3 - 1.5 ng/mL.
  • Rat_CXCL12_SDF-1a_CF_RECOM_1_091118_updated
    Stability testing for rat CXCL12/SDF-1α. Rat SDF-1α was aliquoted in PBS at 0.2 mg/mL and one aliquot was kept at 4°C (control) and another was frozen and thawed four times (4x freeze/thaws). After this procedure, the samples were tested by their property to chemoattract Ba/F3-hCXCR4 transfectants.
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775902 10 µg $170
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775904 25 µg $335
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775906 100 µg $575
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Description

SDF-1 (stromal cell derived factor 1) also known as CXCL12 belongs to the CXCL chemokine family. Six isoforms of SDF-1 has been described: SDF-1α, SDF-1β, SDF-1γ, SDF-1δ, SDF-1ε, SDF-1ϕ. SDF-1 is expressed by many organs, and it is most abundantly expressed in pancreas, spleen, ovary, and small intestine. SDF-1 and its receptor CXCR4 are involved in regulation of migration, survival, and development of multiple cell types, including human hematopoietic CD34+/CD38-/low and stromal STRO-1+ stem cells. Stress-induced modulations in SDF-1 and CXCR4 levels leads to the recruitment of immature and maturing leukocytes from the BM reservoir to damaged organs as part of host defense and repair mechanism. SDF-1/CXCR4 system is involved in the establishment of organ metastasis in different cancers, for example in lymph node metastasis, in breast cancer and oral squamous cell carcinoma (SCC), and peritoneal metastasis in ovarian cancer. SDF-1 also binds to CXCR7, but it does not trigger typical chemokine signal transduction. CXCR7 is a scavenger receptor and regulates the extracellular accessibility of SDF-1. CXCR7 form a dimer with CXCR4 and regulates SDF-1-mediated G protein signaling. CXCR4 and CXCR7 are involved in development as well as in cancer.

Product Details
Technical data sheet

Product Details

Reactivity
Rat
Source
Rat CXCL12 amino acids (Lys22-Lys89) (Accession #Q80YV8) was expressed in E. coli.
Molecular Mass
The 68 amino acid recombinant protein has a predicted molecular mass of approximately 7.9 kD. The DTT-reduced and non-reduced protein migrate at approximately 10kD and 15KD respectively by SDS-PAGE. The predicted N-terminal amino acid is Lys.
Purity
> 95% by SDS-PAGE gel as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS pH 7.2.
Endotoxin Level
Less than 1.0 EU per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 50 µg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 0.2 - 1% BSA or HSA. After dilution, the cytokine can be stored at 4°C for one month or from -20°C to -70°C for up to three months. Avoid repeated freeze/thaw cycles.
Activity
Rat CXCL12/SDF-1α chemoattracts Ba/F3 - hCXCR4 transfectants in a dose dependent manner. The migrated cells were measured using Deep Blue Cell Viability™ Kit (Cat. No. 424701). The ED50 = 0.3 - 1.5 ng/mL.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Dimer
Distribution

Widely expressed in endothelial and stromal cells.

Function
SDF-1 chemo-attracts peripheral blood T, lamina propia T cells, and B cells. The SDF-1/CXCR4 interaction mobilizes CD34+ progenitors to the peripheral blood and participates in the homing of stem cells to their specific niches. SDF-1-induced proliferation in pancreatic intraepithelial neoplasia cells (PanIN). SDF-1 is a constitutive chemokine involved in lung, brain, joint, brain, and intestine inflammation.
Interaction
T lymphocytes, B cells, monocytes, macrophages, tissue-committed stem/progenitor cells (TCSCs), mast cells, and vascular smooth muscle cells (VSMCs).
Ligand/Receptor
CXCR4 and CXCR7
Bioactivity
Rat CXCL12 induces the chemotaxis of Baf3-hCXCR4 trnasfectants.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction, Transcription Factors
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Shirozu M, et al. 1995 Genomics 28:495-500.
  2. Müller A, et al. 2001. Nature. 410:50-6.
  3. Dar A, et al. 2006. Exp. Hematol. 34:967-75.
  4. Burns JM, et al. 2006. J. Exp. Med. 203:2201.
  5. Gelmini S, et al. 2008 J. Endocrinol. Invest. 31:809-19.
  6. Zlotnik A. 2008. J. Pathol. 215:211-3.
  7. Levoye A, et al. 2009. Blood. 113:6085.
  8. Naumann U, et al. 2010. PLoS One. 5:e9175.
Gene ID
24772 View all products for this Gene ID
UniProt
View information about CXCL12 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 1    Revision Date: 09/11/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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