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- Regulatory Status
- RUO
- Other Names
- TNFRSF13B
- Ave. Rating
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- Product Citations
- publications
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Recombinant mouse TACI inhibits the proliferation of activated mouse B cells induced by recombinant mouse BAFF (Cat. No. 591202) in a dose-dependent manner. The ED50 for this effect is 10 – 100 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 577706 | 100 µg | $733 | ||||
| 577708 | 500 µg | $1875 | ||||
Transmembrane activator and calcium-modulator and cytophilin ligand interactor (TACI) is a TNFR family member molecule and is part of the so-called BAFF system. This includes two ligands: B cell-activating factor of the TNF family (BAFF) and a proliferation-inducing ligand (APRIL), and three receptors: BAFFR (TNFRSF13C), TACI (TNFRSF13B), and B cell maturation Ag (BCMA or TNFRSF17). BAFF binds the three receptors, while APRIL binds TACI and BCMA. TACI is constitutively expressed on naïve B cells and is upregulated on activated B cells. TACI levels are severely reduced in newborn mice B cells compared with those of adult mice, and newborn B cells do not secret Igs when they are stimulated with BAFF or APRIL. This reduced TACI expression might be responsible for the irresponsiveness of newborn B cells to T cell independent type II antigens such as bacterial capsular polysaccharides.
Product DetailsProduct Details
- Source
- Mouse TACI (Accession# NM_021349: Met1-Thr 129) - SRENLYFQG - Human IgG (Pro 100 - Lys300) was expressed 293E cells.
- Molecular Mass
- The total predicted molecular weight is 41 kD. The non-reduced protein migrates between 80-90 kD and the DTT-reduced at approximately 45 - 50 kD by SDS-PAGE.
- Purity
- >98%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS
- Endotoxin Level
- Less than 0.01 ng per µg cytokine as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Recombinant mouse TACI inhibits the proliferation of activated mouse B cells induced by recombinant mouse BAFF (Cat. No. 591202) in a dose-dependent manner. The ED50 for this effect is 10 – 100 ng/mL.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
- Product Citations
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Antigen Details
- Structure
- Disulfide linked homodimer
- Distribution
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Peripheral B lymphocytes
- Function
- TACI triggers immunoglobulin class switching, IgG and IgA, by activating B cells through the adaptor MyD88. It also enhances the generation and maintenance of isotype-switched memory B lymphocytes and plasma cells, and increases the affinity of the produced antibody by the process of somatic hypermutation of the Ig gene. TACI drives plasma cell differentiation in LPS activated B cells. In addition, TACI is required in T-independent antibody production and in the regulation of B cell numbers. The interaction between B cells (expressing TACI) and DC (expressing BAFF) induces a rapid DC maturation which primes naive CD8+ T cells.
- Interaction
- B cells, myeloid cells, and monocytes derived dendritic cells.
- Ligand/Receptor
- APRIL (TNFSFL13A, CD256) and BAFF (TNFSFL13B, CD257) are TACI ligands.
- Cell Type
- B cells
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
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1. Schneider P, et al. 1999. J. Exp. Med. 189:1747.
2. Diaz-de-Durana Y, et al. 2006. Blood 107:594.
3. Kanswal S, et al. 2008. J. Immunol. 181:976.
4. Yang J, et al. 2009. PLOS One 4:e8494
5. Ozcan E, et al. 2009. J. Allergy Clin. Immunol. 123:1277.
6. He B, et al. 2010. Nat. Immunol. 1:836. - Gene ID
- 57916 View all products for this Gene ID
- UniProt
- View information about TACI on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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