Recombinant Mouse IL-12 (p70) (carrier-free)

Pricing & Availability
Other Names
Natural killer cell stimulatory factor (NKSF), cytotoxic lymphocyte maturation factor (CLMF).
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Product Citations
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Mouse_IL-12_CF_recom_101119
Mouse IL-12 induces INFγ production in mouse splenocytes in a dose dependent manner. The ED50 = 0.01 to 0.1 ng/ml
  • Mouse_IL-12_CF_recom_101119
    Mouse IL-12 induces INFγ production in mouse splenocytes in a dose dependent manner. The ED50 = 0.01 to 0.1 ng/ml
Cat # Size Price Quantity Avail. Save
577002 10 µg $195
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577004 25 µg $350
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577006 100 µg $1,200
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577008 500 µg $2,600
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Description

IL12 (p70) is a disulfide-linked heterodimer composed of unrelated p40 (glycosylated) and p35 subunits. IL-12 acts as a growth factor for activated human T and NK cells, enhance the lytic activity of human NK cells, and stimulate the production of IFNg, by resting human PBMC. IL-12R is formed by two chains, IL-12Rβ1 and IL-12Rβ2. IL-12Rβ1 is associated with the Janus kinase (Jak) Tyk2 and binds IL-12 p40; IL-12Rβ2 is associated with Jak2 and binds either the heterodimer or the p35 chain. Signaling through the IL-12 receptor complex induces phosphorylation, dimerization, and nuclear translocation of several signal transducer and activator of transcription (STAT) family members (STAT1, 3, 4, 5), but most of the biological responses to IL-12 have been attributed to STAT4.

Product Details
Technical data sheet

Product Details

Source
Expressed in Sf9 cells as secreted protein (p40: Accession# NM_008352, p35: Accession#NM_008351).
Molecular Mass
The mIL-12 consists of two subunits linked via a disulphide bond: P40 (Accession# P43432: Met23-Ser335) and P35 (Accession# NP_032377: Arg23-Ala215). The total predicted molecular weight is 57.48 kD. The non-reduced protein migrates at approximately 55 kD and the DTT-reduced protein produces two bands at approximately 40 kD and 24 kD by SDS-PAGE.
Purity
95%, as determined by Coomassie stained SDS-PAGE.
Formulation
The protein was 0.22 µm filtered in 10 mM NaH2PO4, 150 mM NaCl, pH 7.2.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Mouse IL-12 induces INFγ production in mouse splenocytes in a dose dependent manner. The ED50 = 0.01 to 0.1 ng/ml.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Additional Product Notes

Get a 50% discount on this product when purchased in our Activation Bundles. Restrictions apply. Learn more…

Product Citations
  1. Bremser A, et al. 2015. PLoS One. 10: 0137393. PubMed
  2. Everts B, et al. 2016. J Exp Med. 213: 35 - 51. PubMed
  3. Bao K, et al. 2016. J Immunol. 197(11):4371-4381. PubMed
  4. Xu Y, et al. 2019. JCI Insight. 4:5. PubMed
  5. Huang A, et al. 2019. JCI Insight. 4. PubMed
  6. Ramstead AG, et al. 2020. Cell Rep. 30:2889. PubMed

Antigen Details

Structure
Heterodimer
Function
IL-12 is produced by myeloid cells and DCs in response to microbial stimuli, such as those mediated by bacteria, fungi, viruses, and intracellular parasites. IL-12 drives Th1 differentiation and IFNγ production. IL-12 acts as a bridge between innate resistance and adaptive immunity. IL-12 production by activated APC is suppressed by IL-10. In addition, IL-12 production by macrophages is regulated by TNFα and nitric oxide. TLR-4 and TLR-9 can cooperate to increase selectively IL-12 production by murine dendritic cells.
Interaction
Cells of hematopoietic origin express the IL-12R, including NK cells, activated T-cells and dendritic cells.
Ligand/Receptor
IL-12 receptor is a heterodimer containing IL-12Rβ1 and IL-12Rβ2 subunits.
Cell Sources
IL-12 is produced by monocytes, macrophages, neutrophils, dendritic cells and B cells. In the CNS, astrocytes and microglia are the main sources of IL-12.
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Schoenhaut DS, et al. 1992. J. Immunol.. 148:3433.
2. Manetti R, et al. 1994. J. Exp. Med. 179:1273.
3. Ireland D, et al.2005. Viral Immunol. 18:397.
4. Moreno SE, et al. 2006. J. Immunol. 177:3218.
5. Lyakh L, et al. 2008. Immunol. Rev.. 226:112.
6. Theiner G, et al. 2008. Mol. Immunol. 45:244.

Gene ID
16159 View all products for this Gene ID 16160 View all products for this Gene ID
UniProt
View information about IL-12 p70 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 4    Revision Date: 08/19/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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