Recombinant Mouse FGF-1-acidic (carrier-free)

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Other Names
Fibroblast growth factor 1 (FGF-1, FGF1), Acidic fibroblast growth factor (aFGF), Endothelial cell growth factor (ECGF), Heparin-binding growth factor 1 (HBGF-1), HBGF1, FGF
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Product Citations
publications
FGF-1_Acidic_Mouse_RECOM_BA_033115
NIH3T3 proliferation inducted by Mouse FGF acidic in th presence of 10 µg/ml heparin.
  • FGF-1_Acidic_Mouse_RECOM_BA_033115
    NIH3T3 proliferation inducted by Mouse FGF acidic in th presence of 10 µg/ml heparin.
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Cat # Size Price Quantity Avail. Save
750902 10 µg $75
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750904 25 µg $145
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750906 100 µg $295
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750908 500 µg $895
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Description

FGF acidic, one of the most studied members of the fibroblast growth factor family, is a powerful mitogen that exhibits strong actions on many different cell types. FGF acidic activities can be mediated not only by autocrine/paracrine pathways, but also in intracrine pathways. FGF acidic lacks a secretion signal peptide and is exported through a non-classical pathway. Endogenous FGF acidic is found in the nucleus of most of the cell-types tested. The nuclear localization is required for FGF acidic mitogenic activity. FGF acidic promotes tumor development by promoting cancer cell proliferation and survival. Increased FGF acidic expression in early stages of many different cancers have been reported. MCF-7 breast cells overexpressing FGF acidic can form vascularized and metastatic tumors in ovariectomized or tamoxifen-treated nude mice. FGF acidic also induces angiogenesis in vitro and in vivo. FGF acidic is an attractive candidate for cancer immunotargeting. FGF acidic also plays a role in neuronal cells' differentiation and survival. FGF acidic is highly expressed in motor neurons. In response to damage, motor neurons can release FGF acidic and subsequently astrocyte activation. Astrocytes release FGF acidic under oxidative stress, which stimulates apoE/HDL generation in an autocrine manner for brain protection. The participation of FGF acidic has been reported in inflammation, cardioprotection, wound healing, adipocyte remodeling, and restenosis.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Source
Mouse FGF acidic, amino acids (Phe16-Asp155) (Accession# BC037601), with an N-terminal Met was expressed in E. coli.
Molecular Mass
The 141 amino acid recombinant protein has a predicted molecular mass of approximately 15.9 kD. The protein migrates approximately at 20 kD in both DTT-reducing and non-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is Met.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filterd protein solution is in 20 mM MOPS, 100 mM NaCl, and 0.2 mM EDTA, pH 7.2
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored at -20°C for three months, or at -70°C for six months. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20°C to -70°C. Stock solutions can also be prepared at 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Activity
ED50 = 0.01 - 0.1 ng/ml, corresponding to a specific activity activity of 1.0 X 107 - 10 X 107 units/mg, as determined by a dose-dependent stimulation of NIH3T3 cell proliferation in the presence of 10 µg/ml of heparin. Proliferation was measured by the colormetric MTS assay.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Growth factor.
Distribution

FGF acidic is widely expressed in developing and adult tissues.

Function
FGF acidic plays an important role in many biological processes, which include cell proliferation, inflammation, and angiogenesis. FGF acidic has low stability and a very short half life in vivo. Binding to heparin increases FGF1 stability, and this binding is important for the formation of active FGF acidic/FGFR complex.
Interaction
Intracellular FGF acidic interacts directly with CK2, FIBP, mortalin, and LRRC59 (ribosome-bind protein p34/leucine-rich repeat containing 59).
Ligand Receptor
Cell-surface FGFR isoforms (FGFR1b, 1c, 2b, 2c, 3b, 3c, and 4), heparin, and heparin sulfate proteoglycans on the cell surface.
Cell Type
Neural Stem Cells, Mesenchymal Stem Cells, Hematopoietic stem and progenitors, Embryonic Stem Cells
Biology Area
Cell Biology, Neuroscience, Synaptic Biology, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Gene ID
14164 View all products for this Gene ID
UniProt
View information about FGF-1-acidic on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 3    Revision Date: 09/21/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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