Recombinant Mouse CXCL13 (carrier-free)

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Other Names
B cell attracting chemokine (BCA-1), B-lymphocyte chemoattractant (BLC), ANGIE, ANGIE2, BCA1, BLR1L, SCYB13
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CXCL13_Mouse_Recombinant_Protein_BA_021313
Baf3-mCXCR5 transfectants attracted by mouse CXCL13.
  • CXCL13_Mouse_Recombinant_Protein_BA_021313
    Baf3-mCXCR5 transfectants attracted by mouse CXCL13.
Cat # Size Price Quantity Avail. Save
583902 10 µg $125
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583904 25 µg $215
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583906 100 µg $585
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583908 500 µg $1,525
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Description

CXCL13 was initially described as a chemokine, highly expressed in the follicles of Peyer's patches, spleen, and lymph nodes, and as a strong chomoattractant for B cells. CXCL13 is expressed by T follicular helper cells, dendritic cells, and stromal cells (including marginal reticular cells (MRCs) and follicular dendritic cells (FDCs)) in B cell areas of secondary lymphoid tissue. CXCL13 binds to CXCR5 that was initially cloned from Burkitt’s lymphoma cells and named BLR1.

B cells migrate in lymphoid organs in search of specific antigens. B cells enter lymph nodes through high endothelial venules and migrate through the lymph node follicle in a CXCL13/CXCR5 dependent manner. During the B cell migration in the follicle, they are in contact with FDCs, MRCs, and cells localized around the follicle perimeter, such as sinus-associated macrophages and T cell zone-associated DCs. Subsequent to the migration through the FDC network, the B cells travel through the T cell zone-proximal follicle. In this migration, CCL21, CCL19, CCR7 and CXCR5 are involved. CXCL13, CCL19, and CCL21 take part in the formation of tertiary lymphoid tissues in chronic antigen-induced arthritis.

High expression of CXCL13 has been linked to lupus, Helicobacter pylori-associated gastritis, multiple sclerosis, rheumatoid arthritis, and the allergic airway inflammatory process. In summary, CXCL13 is highly expressed at sites of new lymphoid tissue formation in a variety of chronic inflammatory conditions.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Source
Mouse CXCL13, amino acids Ile22- Ala109 (Accession# NP_061354.1) was expressed in E. coli.
Molecular Mass
The 78 amino acid recombinant protein has a predicted molecular mass of approximately 9.79 kD. The DTT-reduced and non-reduced protein migrate at approximately 15 and 13 kD by SDS-PAGE respectively. The N-terminal amino acid is Ile.
Purity
>98%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored at -20°C or -70°C for six months. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. After dilution, the cytokine can be stored between 2°C and 8°C for one month or from -20°C to -70°C for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Bioactivity was measured by its property to chemoattract BaF3-mCXCR5 transfectants in a dose dependent manner.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Chemokine
Distribution
Folicular dendritic cells, monocytes, macrophages, stromal cells from germinal centers. CXCL13 is derived from endothelial cells, synovial fibroblasts, and follicular dendritic cells in rheumatoid arthritis.
Function
CXCL13 chemoattracts B cells and is a key chemokine for B cell entry to lymphoid follicles. Also, it takes part in the formation of ectopic lymphoid tissue in chronic inflammation. CXCL13 production by follicular Th cells is induced by TCR activation.
Interaction
B cells, follicular helper CD4 T cells
Ligand/Receptor
CXCR5 (CD185)
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Gunn MD, et al. 1998. Nature 391:799.
2. Legler DF, et al. 1998. J. Exp. Med. 187:655.
3. Kim CH, et al. 2004. Blood 104:1952.
4. McDonald KG et al. 2010. Am. J. Path. 176:2367.
5. Cyster GJ, 2010. Nature Immunol. 11:989.
6. Gatto D, et al. 2011. J. Immunol. 187;4621.

Gene ID
55985 View all products for this Gene ID
UniProt
View information about CXCL13 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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