Recombinant Mouse CCL25 (TECK) (carrier-free)

Pricing & Availability
Other Names
TECK (Thymus-Expressed Chemokine), Small-inducible cytokine A25 (SCYA25), CKb15 (Ck beta-15)
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Product Citations
publications
CCL25_Mouse_Recombinant_Protein_BA_041913
Baf3-hCCR9 transfectants chemoattracted by mouse CCL25.
  • CCL25_Mouse_Recombinant_Protein_BA_041913
    Baf3-hCCR9 transfectants chemoattracted by mouse CCL25.
Cat # Size Price Quantity Avail. Save
589302 10 µg $125
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589304 25 µg $215
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589306 100 µg $585
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589308 500 µg $1,525
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Description

CCL25, which signals through CCR9 receptor, is mainly expressed in the thymus and small intestine. In the thymus, CCL25 contributes to correct thymocyte maturation by promoting CD4+CD8+ thymocyte trafficking through the cortex via α4β1-dependent adhesion to VCAM-1, and also stimulates CD4+CD8- cell attachment to fibronectin in the medulla. In the small intestine, CCL25 is expressed by intestinal epithelium and controls segmental specialization of the intestinal immune system by recruiting gut-specific memory/effector T cells.

CCL25 also promotes melanoma, ovarian cancer, breast cancer, leukemia, and prostatic carcinoma cell migration. A recent report shows that multiple myeloma cells can produce CCL25 and induce mesenchymal stromal cell migration. Bone marrow pre-pro-B cells also migrate in response to CCL25. CCL25 expression can be induced in the liver of patients with primary sclerosing cholangitis. Intestinal epithelial cell expression of CCL25 is also reduced in HIV-positive individuals.
 

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Source
Mouse CCL25, amino acids Gln24-Asn144 (Accession# O35903.2) was expressed in E. coli.
Molecular Mass
The 121 amino acid recombinant protein has a predicted molecular mass of approximately 16 kD. The DTT-reduced and non-reduced protein migrate at approximately 16 kD by SDS-PAGE. The N-terminal amino acid is Glutamine.
Purity
>98%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20°C to -70°C. Stock solutions can also be prepared at 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Activity
Bioactivity was measured by its ability to chemoattract Baf3-hCCR9 cells in a dose dependent manner.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
CC Chemokine
Distribution

CCL25 is expressed by small intestinal epithelial cells, thymic medullary dendritic cells, and cortex epithelial cells.

Function
CCL25 is a chemokine that is involved in T cell development in the thymus and in recruiting effector lymphocytes to small intestinal epithelium. CCL25 induces extravasation of IgA antibody-secreting cells in the small intestine.
Interaction
CD4 and CD8 single positive thymocytes, immature double positive thymocytes, macrophages, dendritic cells, IgA antibody-secreting cells, and cancer cells.
Ligand/Receptor
CCR9
Cell Type
B cells, Dendritic cells, Tregs
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Hieshima K, et al. 2004. J. Immunol. 173:3668.

Gene ID
20300 View all products for this Gene ID
UniProt
View information about CCL25 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 4    Revision Date: 09/12/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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