Recombinant Mouse Cathepsin B (CTSB) (carrier-free)

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Other Names
CTSB, CPSB, APP secretase, APPS, Cathepsin B1, CB
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Mouse_CathepsinB_CF_RECOM_BA_011217
Mouse CTSB enzyme activity was measured using a fluorogenic peptide substrate Z-Leu-Arg-AMC.
  • Mouse_CathepsinB_CF_RECOM_BA_011217
    Mouse CTSB enzyme activity was measured using a fluorogenic peptide substrate Z-Leu-Arg-AMC.
Cat # Size Price Quantity Avail. Save
763002 10 µg $190
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763004 25 µg $375
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763006 100 µg $995
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763008 500 µg $2,995
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Description

Cathepsin B, also known as CTSB, is a lysosomal cysteine protease. While most cathepsins are exclusively endopeptidases, CTSB exhibits both carboxypeptidase and endopeptidase activities. The optimal pH for CTSB activity is between four and six. Cystatin C has been identified as an endogenous CTSB inhibitor. High CTSB protein levels and activities have been found in many tumors including breast, cervix, colon, stomach, glioma, lung, and thyroid tumors. CTSB can be secreted by tumor cells and is associated with the cell membrane of these cells. Membrane associated CTSB promotes extracellular matrix (ECM) degradation, which contributes to cancer motility and invasion. Many ECM proteins, including laminin, fibronectin, and collagen IV, are substrates of CTSB. CTSB can also activate pro-uPA/PLAU. Activated uPA promotes ECM digestion through serine protease plasminogen. It has been shown that the inhibition of CTSB can limit bone metastasis in breast cancer, making it an important anti-cancer drug target. CTSB has been proposed as a new drug target for Alzheimer's disease because of its involvement in the production of neurotoxic β-amyloid (Aβ) peptides. The inhibition of CTSB can reduce the brain Aβ peptides and improve memory which was determined from a mouse model with Alzheimer's disease. CTSB also plays significant roles in immune responses including both T and B cell apoptosis and Th1/Th2 polarization. CTSB is implicated in other pathological conditions including cardiovascular disease, multiple sclerosis, and arthritis. The roles of CTSB in autophagy, adipogenesis, and cholesterol absorption in the intestine have also been studied.

Product Details
Technical data sheet

Product Details

Source
Mouse Cathepsin B, amino acids (His18-Phe339) (Accession# P10605), with C-terminal 10x His tag, was expressed in CHO cells.
Molecular Mass
The 332 amino acid recombinant protein has a predicted molecular mass of approximately 36.7 kD. The protein migrates at approximately 45 kD in DTT-reducing conditions and at approximately 40 kD in non-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is His.
Purity
>95%, as determined by Coomassie stained SDS-PAGE under reducing conditions.
Formulation
0.22 µm filtered protein solution is in 25 mM Tris, 150 mM NaCl, and pH 7.5.
Endotoxin Level
Less than 0.01 ng per µg cytokine, as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored at -20°C for one month, or at -70°C for six months. For maximum results, quick spin vial prior to opening. Avoid repeated freeze/thaw cycles.
Activity
The activity of Cathepsin B is determined by its ability to cleave the fluorogenic peptide substrate, Z-Leu-Arg-AMC (Z=Benzyloxycarbonyl, and AMC=7-amino-4-methylcoumarin) after activation. The specific activity is > 3600 pmol/min/µg.
Application

Bioassay

Application Notes

Cathepsin B Activity Protocol:

1. Dilute the recombinant mouse Cathepsin B to 10 μg/ml in activation buffer (25 mM MES, 1 mM DTT, pH 5.0) and incubate the protein at 25°C for 3 min. Over activation may result in proteolysis and low activity.
2. Dilute the recombinant mouse Cathepsin B to 0.05 μg/ml in assay buffer (25 mM MES, pH 5.0).
3. Dilute the fluorogenic peptide substrate (Z-LR-AMC) to 40 μM using assay buffer.
4. Perform the assay in a 96-well, black, flat-bottomed plate. Add 50 μl of the diluted, activated Cathepsin B (0.0025 µg/well) to a black well and start the reaction by adding 50 μl of 40 μM substrate. The assay is read on a fluorometer at excitation and emission wavelengths of 380 nm and 460 nm for five minutes (top read).

This protein is in the latent form and needs to be activated for bioassay.



BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Cysteine protease.
Distribution
CTSB is ubiquitously expressed and is normally localized to lysosomes. However, it can also be secreted by many cells including tumor cells, macrophages, fibroblasts, osteoclasts, T lymphocytes, and neutrophils.
Function
Cathepsin B is a lysosomal cysteine protease that is normally involved in intracellular protein turnover.
Interaction
Interacts with Hepatitis B spliced protein, Bikunin, and TSRC1.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance, Stem Cells
Molecular Family
Enzymes and Regulators, Lysosomal Markers
Antigen References

1. Mohamed MM, Sloane BF. 2006. Nat. Rev. Cancer. 6:764.
2. Wang C, et al. 2012. J. Biol. Chem. 47:39834.
3. Grzonka Z, et al. 2001. Acta Biochim. Pol. 48:1.
4. Liu J, et al. 2006. FEBS Letters 580:245.
5. Brix K, et al. 2008. Biochimie. 90:194.
6. Turk V, et al. 2012. Biochim. Biophys. Acta.1824:68.

Gene ID
13030 View all products for this Gene ID
UniProt
View information about Cathepsin B on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 1    Revision Date: 01/12/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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