- Other Names
- Lymphotactin-2, SCYC2, SCM1B, SCM-1 beta
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- Product Citations
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XCL2, also known as lymphotactin-2, is an inflammatory chemokine secreted by T cells and natural killer (NK) cells. XCL2 was initially identified as a variant form of XCL1 that showed only two amino acid differences. XCL1 and XLC2 are structurally similar and share similar agonistic functions. Under physiological conditions, the conformation of XCL1 and XCL2 transitions between the conserved chemokine folds and an unrelated dimeric structure. The monomer form of XCL2 is responsible for the activation of its cognate receptor XCR1, while the dimeric form is important for glycosaminoglycan (GAG) binding. The binding of XCL2 to XCR1 initiates chemotactic cell migration in dendritic cells as well as in T and B lymphocytes. In cancers such as epithelial ovarian and hepatocellular carcinomas, the increased XCL2 expression correlates with cancer progression. Human XCL2 encodes a 114 amino acid precursor protein with a 21 amino acid signal peptide and a single disulfide bond.Product Details
- Human XCL2, amino acids (Val22-Gly114) (Accession# Q9UBD3), was expressed in E. coli.
- Molecular Mass
- The 93 amino acid recombinant protein has a predicted molecular mass of approximately 10.3 kD.
- >98%, by SDS-PAGE, HPLC, MALDI-MS, and NMR.
- Lyophilized powder.
- Endotoxin Level
- Less than 1.0 EU per µg of protein as determined by the LAL method.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1 - 0.5 mg/mL. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1% BSA and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- ED50 = 0.02 - 0.1 µg/mL as measured by its ability to chemoattract BaF3 mouse pro B cells transfected with human XCR1.
- Monomer and Dimer.
- Spleen, peripheral leukocytes, CD4+ and CD8+ T cells, and natural killer (NK) cells.
- T cell and NK cell recruitment.
- T cell, NK cell, and Dendritic cells.
- Ligand Receptor
- Chemoattract BaF3 mouse pro B cells transfected with human XCR1.
- Biology Area
- Molecular Family
- Antigen References
1. Kennedy J, et al. 1995. J. Immunology 155:203-209.
2. Yoshida T, et al. 1996. FEBS Lett. 395:82-88.
3. Fox JC, et al. 2015. Cytokine 71(2):302-311.
4. Volkman BF, et al. 2009. Methods Enzymol. 461:51-70.
5. Lei Y, Takahama Y. 2012. Microbes Infect. 14(3):262-7.
6. Guzzo C, et al. 2013. PLoS Pathog. 9(12):e1003852.
7. Kroczek RA, Henn V. 2012. Front Immunol. 3:14.
- Gene ID
- 6846 View all products for this Gene ID
- View information about XCL2 on UniProt.org
- Does specific activity of a recombinant protein vary between lots?
Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- Have your recombinants been tested for stability?
Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
- Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
- How does the activity of your recombinant proteins compare to competitors?
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.