- Regulatory Status
- Other Names
- Vascular Adhesion Protein-1 (VAP-1), AOC3, SSAO, HPAO, Copper Amine Oxidase, Membrane Primary Amine Oxidase
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- Product Citations
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VAP-1 is a type II cell adhesion membrane protein belonging to the copper/topaquinone oxidase family. Functionally, VAP-1 is a nonclassical inflammation-inducible endothelial molecule involved in leukocyte-subtype-specific rolling under physiological shear. Molecularly, VAP-1 belongs to a special class of cell surface amino oxidases. The enzymatic reaction itself and the biologically active end products can potentially regulate the adhesive status of the vessel wall. Thus, VAP-1 is an ectoenzyme that has inter-related adhesive and enzymatic functions in regulating physiological trafficking and inflammation. Inhibition of VAP-1 can protect against inflammation related damage to certain injured tissues. This protein is primarily expressed on the high endothelial venules of peripheral lymph nodes and on hepatic endothelia. Additionally, VAP-1 can function as a significant prognostic marker for certain cancers and cardiovascular diseases.Product Details
- Human VAP-1, amino acids (Gly27-Asn763) (Accession# NP_001264660.1), was expressed in CHO cells.
- Molecular Mass
- The 737 amino acid recombinant protein has a predicted molecular mass of approximately 84 kD. Recombinant VAP-1 is a mixture of monomers and disulfide linked homodimers. The predicted N-terminal amino acid is Gly.
- >98%, as determined by Coomassie stained SDS-PAGE.
- Endotoxin Level
- Less than 0.1 ng per µg of protein.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1% BSA and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
- Human VAP-1 is able to produce hydrogen peroxide during the oxidation of benzylamine. The specific activity is > 16 pMoles/min/µg of VAP-1.
- VAP-1 plays an important role in the migration of lymphocytes to inflamed tissue and is a prognostic marker for certain cancers and cardiovascular diseases.
- Human VAP-1 is able to induce production of hydrogen peroxide during the oxidation of benzylamine.
- Biology Area
- Cell Adhesion, Cell Biology, Signal Transduction
- Antigen References
1. Jalkanen S. and Salmi M. 1993. Res. Immunol. 144:746.
2. Pietruczuk M. et al. 2006. Przegl. Lek. 63:220.
3. Jalkanen S. and Salmi M. 2008. Arterioscler. Thromb. Vasc. Biol. 28:18.
- Gene ID
- 8639 View all products for this Gene ID
- View information about VAP-1 on UniProt.org
- Why choose BioLegend recombinant proteins?
• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins.
- How does the activity of your recombinant proteins compare to competitors?
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)