Recombinant Human ICAM-3-Fc Chimera (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
Intercellular adhesion molecule 3, CD50
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Product Citations
publications
Human_ICAM-3_Fc_Chimaera_CF_RECOM_1_020618
Immobilized recombinant human ICAM-3 induces adhesion of retinoic acid (2 µM, 72 hr) activated HL-60 cells in a dose dependent manner. ED50 for this effect is 0.5 – 2.0 µg/mL.
  • Human_ICAM-3_Fc_Chimaera_CF_RECOM_1_020618
    Immobilized recombinant human ICAM-3 induces adhesion of retinoic acid (2 µM, 72 hr) activated HL-60 cells in a dose dependent manner. ED50 for this effect is 0.5 – 2.0 µg/mL.
  • Human_ICAM-3_Fc_Chimaera_CF_RECOM_2_020618
    Stability testing for human ICAM-3. Human ICAM-3 was aliquoted in PBS, pH 7.2 at 0.2 mg/ml. One aliquot was freeze and thawed four times (4x freeze/thaws), and compared to a control kept at 4°C (control). The samples were tested for their ability to induce adhesion of HL-60 cells.
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771504 25 µg $141
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771508 500 µg $1289
 
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Cat. No. 771508 is scheduled for discontinuation. Please use the availability checker to determine current availability. The 25 µg size is available as an alternative.
Description

Intercellular Adhesion Molecule-3 (ICAM-3, CD50) is a highly glycosylated protein that belongs to immunoglobulin superfamily.  It has five extracellular Ig-like domains and binds the leukocyte integrins LFA-1 (CD11a/CD18) and αdβ2.  ICAM-3 is broadly expressed on leukocytes, endothelial cells, and Langerhans cells.  ICAM-3 is an adhesion molecule with signal transducing functions involved in immune responses.  LFA-1/ICAM-3 interactions between APC and resting T cells provide costimulatory signals that are important for initiating immune responses.  LFA-1/ICAM-3 interactions can also exert inhibitory effects on proliferating T cells.  ICAM-3 induces intracellular Ca2+ mobilization and undergoes phosphorylation on tyrosine residue leading to association with the tyrosine phosphatase, CD45.  Soluble forms of ICAM-3 have been found to be elevated in the circulation of patients with autoimmune disorders.  Alternative splicing results in multiple transcript variants encoding different isoforms. Diseases associated with ICAM3 include Actinic Prurigo and Trichosporonosis.

Product Details
Technical data sheet

Product Details

Source
Human ICAM-3, amino acids Gln30-His485 (Accession # BC058903) with a C-terminal human IgG1 Fc-6His tag was expressed in CHO cells.
Molecular Mass
The 701 amino acid recombinant protein has a predicted molecular mass of approximately 77.2 kD. The DTT-reduced protein migrates at approximately 110 kD and non-reduced protein migrate at approximately 220 kD by SDS-PAGE. The predicted N-terminal amino acid is Gln.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2
Endotoxin Level
Less than 1.0 EU per µg protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
ED50= 0.5 - 2.0 µg/mL as measured by the ability of immobilized protein to support the adhesion of retinoic acid (2 µM, 72 hr) activated HL-60 cells.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Homodimer
Distribution

ICAM-3 is broadly expressed on leukocytes, endothelial cells, and Langerhans cells

Function
ICAM-3 is ligand for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2) and integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, it contributes to apoptotic neutrophil phagocytosis by macrophages.
Interaction
Leukocytes, endothelial cells
Ligand/Receptor
LFA-1, integrin α-D/β-2
Bioactivity
Measured by the ability of immobilized protein to support the adhesion of retinoic acid activated HL-60 cells.
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Soluble Receptors
Antigen References
  1. Fawcett JH, et al. 1992. Nature. 360:481-484.Vazeux RH, et al. 1992. Nature 360:485-488.
  2. Bossy D, et al. 1994. Genomics. 23:712-713.
  3. Van der Vieren M, et al. 1995. Immunity. 3:683-690.
  4. Starling GC, et al. 1995. Eur J Immunol. 25:2528-2532.
  5. Kristof E, et al. 2013. Apoptosis. 18:1235-1251.
Gene ID
3385 View all products for this Gene ID
UniProt
View information about ICAM-3 on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
Go To Top Version: 0    Revision Date: 02/12/2018

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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