Recombinant Human CXCL17 (VCC-1) (carrier-free)

Pricing & Availability
Other Names
VEGF co-regulated chemokine 1 (VCC-1)
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Product Citations
publications
CXCL17_Human_Recombinant_Protein_BA_111912
Bactericidal activity of human CXCL17 against E.coli.
  • CXCL17_Human_Recombinant_Protein_BA_111912
    Bactericidal activity of human CXCL17 against E.coli.
Cat # Size Price Quantity Avail. Save
585302 10 µg $155
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585304 25 µg $285
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585306 100 µg $615
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585308 500 µg $1,785
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Description

CXCL17 is the latest identified member of the CXC chemokine family and may contribute to tumor angiogenesis and progression. Ectopic expression of human CXCL17 in HUVEC significantly upregulated the RNA level of genes involved in angiogenesis including VEGF-A and basic FGF. NIH3T3 cells overexpressing human or mouse CXCL17 developed rapid growing tumors in mice. In vitro chemotaxis assay shows that human CXCL17 can induce migration of CD14+ monocytes and CD11c+ immature dendritic cells from human peripheral blood, and mouse CXCL17 can induce CD11b+Gr1highF4/80 cells from mouse spleen cells. CXCL17 also shows broad antimicrobial activity by disrupting bacterial membrane. High levels of CXCL17 have been detected in bronchoalveolar lavage fluid of patients with idiopathic pulmonary fibrosis.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Source
Human CXCL17, 96 amino acids Leu24-Leu119 (Accession# NP_940879) was expressed in E. coli.
Molecular Mass
The 96 amino acid recombinant protein has a predicted molecular mass of approximately 11.3 kD. The DTT-reduced protein migrates at approximately 11.3 kD and non-reduced protein migrates at approximately 24 kD by SDS-PAGE. The N-terminal amino acid is Leucine.
Purity
>98%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20°C to -70°C. Stock solutions can also be prepared at 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Activity
Bioactivity was measured by its antimicrobial activity (E.coli) in a dose dependent manner.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Application References

(PubMed link indicates BioLegend citation)
  1. Pisabarro MT, et al. 2006. J. Immunol. 176:2069.
  2. Weinstein EJ, et al. 2006. Biochem. Biophys. Res. Commun. 350:74.
  3. Burkhardt AM, et al. 2012. J. Immunol. 188:6399.
  4. Matsui A, et al. 2012. PLoS One. 7:e44080.

Antigen Details

Structure
CXC Chemokine
Distribution
CXCL17 is a mucosal expressed chemokine, and its RNA has been detected in the respiratory system, digestive system, and reproductive tract. In the gut, CXCL17 is expressed by epithelial cells.
Function
CXCL17 chemoattracts monocytes and immature dendritic cells. A transcriptional microarray experiment shows co-regulation of CXCL17 with three other genes involved in angiogenesis (VEGF, IL-8, GRO1). CXCL17 was found to be significantly upregulated in various breast carcinoma and colon tumors.
Ligand/Receptor
Unknown
Cell Type
Dendritic cells
Biology Area
Angiogenesis, Cell Biology, Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Gene ID
284340 View all products for this Gene ID
UniProt
View information about CXCL17 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 2    Revision Date: 06/24/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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