- Other Names
- Poliovirus receptor-like 2, PVRL2, Herpesvirus entry mediator B, HVEB, Poliovirus receptor-related 2, PRR2, CD112 antigen, Nectin2, Nectin-2
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- Product Citations
CD112/Nectin-2, also known as poliovirus receptor related protein 2 (PVRL2), is a Ca2+ independent immunoglobulin-like cell adhesion molecule that belongs to Nectin family. Nectins were originally identified as virus receptors and the family consists of four members, nectin-1, nectin-2 , nectin-3 and nectin-4. Nectins-1, nectin-2, and nectin-3 were named PVRL1, PVRL2, and PVRL3 and assigned as CD111, CD112, and CD113, respectively. Nectins mediate both homophilic and heterophilic interactions. They first form cis-homodimers followed by dimer formation in trans. The trans-interaction can be either homophilic or heterophilic. CD112/Nectin-2 also binds to DNAM-1/CD226 on NK cells. The interaction between CD112 and CD226 can induce NK cell and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112/Nectin-2 also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus. CD112/Nectin-2 localizes to adherens junctions between neurons, endothelial cells, epithelial cells, and fibroblasts.Product Details
- Human CD112, amino acids Gln32-Ala353 (Accession # NM_0010427) with a C-terminal human IgG1 Fc and 6-His tag was expressed in 293E cells.
- Molecular Mass
- The 566 amino acid recombinant protein has a predicted molecular mass of approximately 62.3 kD. The DTT-reduced protein migrates at approximately 70 kD and non-reduced protein migrates at approximately 140 kD by SDS-PAGE. The predicted N-terminal amino acid is Gln.
- > 95%, as determined by Coomassie stained SDS-PAGE.
- 0.22 µm filtered protein solution is in PBS, pH 7.2.
- Endotoxin Level
- Less than 0.1 EU per µg protein as determined by the LAL method.
- 10-25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are bottled at the concentration indicated on the vial.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- When human CD112R is immobilized at 1.0 µg/mL, human CD112 binds with EC50 of 25 - 100 ng/mL.
- Application Notes
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at email@example.com.
Localizes to adherens junctions between neurons, endothelial cells, epithelial cells, and fibroblasts.
- Modulator of T-cell signaling. Can be either a costimulator or a coinhibitor of T-cell function. Upon binding to CD226, it stimulates T-cell proliferation and cytokine production. Upon interaction with PVRIG, it inhibits T-cell proliferation.
- Neurons, endothelial cells, epithelial cells, fibroblasts.
- Measured by its ability to bind human CD112R.
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors, Soluble Receptors
- Antigen References
- Morrison ME, Racaniello VR. 1992. J. Virol. 2807-13.
- Warner MS, et al. 1998. Virology. 179-89.
- Bottino C, et al. 2003. J. Exp. Med. 557-67.
- Liu J, et al. 2012. J. Immunol. 5511-20.
- Zhu Y, et al. 2016. J. Exp. Med. 167-76.
- Gene ID
- 5819 View all products for this Gene ID
- View information about CD112 on UniProt.org
- Does specific activity of a recombinant protein vary between lots?
Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- Have your recombinants been tested for stability?
Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
- Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
- How does the activity of your recombinant proteins compare to competitors?
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.