Recombinant Human CCL25 (TECK) (carrier-free)

Pricing & Availability
Other Names
TECK (Thymus-Expressed Chemokine), Small-inducible cytokine A25 (SCYA25), CKb15 (Ck beta-15)
Ave. Rating
Submit a Review
Product Citations
publications
CCL25_Human_Recombinant_Protein_BA_031813
Baf3-hCCR9 transfectants chemoattracted by human CCL25.
  • CCL25_Human_Recombinant_Protein_BA_031813
    Baf3-hCCR9 transfectants chemoattracted by human CCL25.
Cat # Size Price Quantity Avail. Save
586802 10 µg $125
Check Availability


Need larger quantities of this item?
Request Bulk Quote
586804 25 µg $215
Check Availability


Need larger quantities of this item?
Request Bulk Quote
586806 100 µg $585
Check Availability


Need larger quantities of this item?
Request Bulk Quote
586808 500 µg $1,525
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CCL25 (TECK) binds to CCR9, the only known receptor for TECK. CCR9 is expressed by a small population of circulating memory CD4+ and CD8+ lymphocytes expressing the intestinal homing receptor α4β7. Therefore, CCL25 is a key regulator for CCR9+CD4+ α4β7 T cell homing to the small intestine. In addition, CCL25 induces adhesion of CD103+ (αEβ7) on CD8 intraepithelial lymphocytes to its epithelial ligand E-cadherin.

In inflammatory bowel diseases (IBD), as in celiac and Crohn’s diseases, the proportion of CCR9+ cells in the peripheral blood and in the gut seems to be inversed; there is a lower frequency of CCR9+CD4+ T cells in the gut mucosa than in the peripheral blood. Also, a decrease in CCR9+CD4+ T cells occurs in the gut of HIV-infected patients. This decrease correlates with a lower expression of CCL25 by inflamed gut endothelial cells. Unusual expression of CCL25 in the liver occurs in primary sclerosis cholangitis (extra-intestinal complication of IBD). In this way, human T cells activated in the gut can be recruited to liver endothelium. Functional expression of CCR9 has been detected in melanoma derived cells, and it has been associated to small intestine metastatic melanoma processes. In addition, CCR9 is expressed by ovarian, breast, and prostate cancer cells.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Source
Human CCL25, amino acids Met-Gln24-Leu150 (Accession# NM_005624.3) was expressed in E. coli.
Molecular Mass
The 128 amino acid recombinant protein has a predicted molecular mass of approximately 14.3 kD. The DTT-reduced and non-reduced protein migrate at approximately 16 and 17 kD by SDS-PAGE respectively. The N-terminal amino acid is Met.
Purity
>98%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1% BSA or HSA. After dilution, the cytokine can be stored between 2°C and 8°C for one month or from -20°C to -70°C for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Bioactivity was measured by its property to chemoattract BaF3-hCCR9 transfectants in a dose dependent manner.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
CC Chemokine
Distribution
CCL25 is expressed by thymic dendritic cells, thymic epithelial cells, small intestinal epithelial cells, colon epithelial cells, and venular endothelial cells in small intestine.
Function
CCL25 chemoattracts T cells, macrophages, and dendritic cells. CCL25 participates in T cell homing to the small intestine and induces T-cell progenitor migration in the thymus.
Interaction
CD4 and CD8 single positive thymocytes, immature double positive thymocytes, macrophages from rheumatoid sinovium, dendritic cells (DCs), immature plasmacytoid DCs, IgA antibody-secreting cells, and cancer cells.
Ligand/Receptor
CCR9
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Vicari AP, et al. 1997. Immunity 7:291.
2. Zaballos A, et al. 1999. J. Immunol. 162:5671.
3. Ericsson A, et al. 2004. Eur. J. Immunol. 34:2720.
4. Eksteen B, et al. 2004. J. Exp. Med. 200:1511.
5. Olaussen RW, et al. 2007. Gastroenterology 132:2371.
6. Amersi FF, et al. 2008. Clin. Cancer Res. 14:638.
7. Mavigner M, et al. 2011. J. Clin. Invest. 122:62.
8. Johnson EL, et al. 2010. World J. Surg. Oncol. 8:62.

Gene ID
6370 View all products for this Gene ID
UniProt
View information about CCL25 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 2    Revision Date: 09/12/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

Login/Register
Forgot your password? Reset Password
Request an Account