Recombinant Human CCL14 (HCC-1) (carrier-free)

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Other Names
Hemofiltrate chemokine 1 (HCC1), New CC chemokine 2 (NCC2), Small inducible cytokine subfamily A, member 14 (SCYA14)
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CCL14_Human_Recombinant_Protein_BA_040513
THP-1 cells chemoattracted by human CCL14.
  • CCL14_Human_Recombinant_Protein_BA_040513
    THP-1 cells chemoattracted by human CCL14.
Cat # Size Price Quantity Avail. Save
587202 10 µg $170
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587204 25 µg $260
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587206 100 µg $595
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587208 500 µg $1,695
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Description

CCL14 is a homeostatic chemokine present at high concentrations in normal human plasma. CCL14 shares 46% sequence identity with CCL3 and CCL4, and it is a weak CCR1 agonist. The NH2- terminus of CCL14 can be cleaved by urokinase plasminogen activator and/or plasmin, and the resulting CCL14 (9-74) chemokine becomes a strong agonist for CCR1, CCR5, and a weak agonist for CCR3. Subsequently, CCL14 (9-74) is a substrate for dipeptidyl peptidase (CD26) that removes two amino acids to produce CCL14 (11-74), which is inactive. CCL15 and CCL23 similarly have an extended N-terminal domain that is cleaved, which activates them. CCL14, in conjuction with CX3CL1 and CCL4, promotes trophoblast migration at the feto-maternal interface. In addition, it has been published that modifying CCL14, eliminating amino acids 1-8 and substituting amino acid in position 9 by nonanoic acid, led to the identification of a potent CCR3 agonist, n-nonanoyl (NNY)-CCL14 (10-74). NNY-CCL14 induces release of reactive oxygen species and leads to chemotaxis of human eosinophils. Also, it acts as an agonistic inactivator of CCR1, CCR2, CCR3, and CCR5. CCL14 is constitutively expressed in different tissues, and its concentration in plasma increases up to 10-fold in inflammatory conditions and in chronic renal failure.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Source
Human CCL14, amino acids Met-(Gly28-Asn93) (Accession# Z49270) was expressed in E. coli.
Molecular Mass
The 67 amino acid recombinant protein has a predicted molecular mass of approximately 7.9 kD. The DTT-reduced and non-reduced protein migrate at approximately 11 and 17 kD by SDS-PAGE respectively. The N-terminal amino acid is Met.
Purity
>98%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1% BSA or HSA. After dilution, the cytokine can be stored between 2°C and 8°C for one month or from -20°C to -70°C for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Bioactivity was measured by its property to chemoattract THP-1 cells in a dose dependent manner.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Distribution
CCL14 is expressed in a large variety of tissues.
Function
CCL14 (9-74) chemoattracts T lymphoblasts, monocytes, eosinophils, and human trophoblasts. CD26 inactivates CCL14 (9-74) and D6 mediates its degradation.
Interaction
Monocytes, macrophages, T lymphocytes, and eosinophils
Ligand/Receptor
CCR1, CCR3, and CCR5
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure
Molecular Family
Cytokines/Chemokines
Antigen References

1. Schulz-Knappe P, et al. 1996. J. Exp. Med. 183:295.
2. Detheux M, et al. 2000. J. Exp. Med. 192:1501.
3. Vakili J, et al. 2001. J. Immunol. 167:3406.
4. Hannan NJ, et al. 2006. Biol. Reprod. 74:896.
5. Savino B, et al. 2009. J. Biol. Chem. 284:26207.
6. Gupta S, et al. 2010. J. Leukoc. Biol. 88:383.

Gene ID
6358 View all products for this Gene ID
UniProt
View information about CCL14 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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