Recombinant Human B7-H1 (PD-L1, CD274)-Fc Chimera (carrier-free)

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Other Names
CD274, Programmed cell death 1 ligand 1 (PD-L1), B7 homolog 1 (B7h1)
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Product Citations
publications
Human_B7-H1_RECOM_BA_102516
Immobilized human PD-L1 binds human PD-1 in a dose dependent manner.
  • Human_B7-H1_RECOM_BA_102516
    Immobilized human PD-L1 binds human PD-1 in a dose dependent manner.
Cat # Size Price Quantity Avail. Save
762504 20 µg $75
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762506 100 µg $185
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Description

PD-L1 is a type I transmembrane protein of 290 amino acids, and it is a member of the B7 family. Human PD-L1 has 70% amino acid identity to its mouse orthologue. Binding of PD-L1 to its receptor PD-1 leads to the inhibition of T cell receptor–mediated lymphocyte proliferation and cytokine secretion. PD-L1 induces IL-10 production in T cells stimulated with low levels of anti-CD3. PD-L1/PD-1 interaction suppresses immune responses against autoantigens and tumors and plays an important role in the maintenance of peripheral immune tolerance. Disruption of the PD-L1 gene leads to up-regulated T cell responses and the generation of self-reactive T cells. Antibodies against PD-1 or PD-L1 leads to increased antitumor immunity. PD-L1 has an important role in conferring fetomaternal tolerance in an allogeneic pregnancy model; antibodies against PD-L1 lead to a breakdown in maternal tolerance to the fetus. PD-L1 shares its receptor with PD-L2 (CD273, B7-DC). PD-L2 has a more limited expression than PD-L1, being expressed on activated macrophages and dendritic cells. PD-L1 is expressed in many tumors, and the interaction with its receptor activates signaling pathways that inhibit T-cell activity and therefore the antitumor immune response. Antibodies targeting PD-1 or PD-L1 block the PD-1 pathway and reactivate T cell activity.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Source
Human B7-H1, amino acids (Phe19-Arg238) (Accession# NP_054862) was expressed in CHO cells. Human IgG-Fc is in the carboxy-terminus.
Molecular Mass
The 453 amino acid recombinant protein has a predicted molecular mass of approximately 51.2 kD. The predicted N-terminal amino acid is Phe.
Purity
>98% as determined by Coomassie stained SDS-PAGE and HPLC analyses.
Formulation
Lyophilized from 10mM Sodium Phosphate + 25 mM NaCl, pH 7.6.
Endotoxin Level
Less than 0.1 ng per µg of protein (<1.0 EU/µg), as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored at -20°C or -70°C for twelve months. For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1 - 1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Activity
Immobilized human PD-L1 binds human PD-1 in a dose dependent manner. The ED50 = 0.2 - 0.8 µg/ml.
Recommended Usage

Bioassay

Product Citations
  1. Kim A, et al. 2019. J Immunol. 203:1076. PubMed

Antigen Details

Structure
Dimer.
Distribution

APC, monocytes, dendritic cells, expressed in nonlymphoid tissues, and stromal cell.

Function
Enhances CD28-independent T-helper cell function, suppresses immune responses against autoantigens, and participates in fetomaternal tolerance.
Interaction
Antigen-stimulated T and B cells, regulatory T cells, follicular T and B cells, dendritic cells, and monocytes.
Ligand/Receptor
PD-1 (CD279).
Bioactivity
Immobilized human PD-L1 binds its receptor PD-1.
Cell Type
Embryonic Stem Cells
Biology Area
Immunology, Cancer Biomarkers, Costimulatory Molecules, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors, Soluble Receptors
Antigen References

1. Dong H, et al. 1999. Nat. Med. 5:1365.
2. Freeman GJ, et al. 2000. J. Exp. Med. 192:1027.
3. Latchman Y, et al. 2001. Nat. Immunol. 2:261.
4. Latchman YE, et al. 2004. Proc. Natl. Acad. Sci. USA 101:10691.
5. Guleria I, et al. 2005. J. Exp. Med. 202:231.
6. Lin DY, et al. 2008. Proc. Natl. Acad. Sci. USA 105:3011.
7. Dai S, et al. 2014. Cell Immunol. 290:72.
8. Melero I, et al. 2015. Nat. Rev. Cancer 15:457.

Gene ID
29126 View all products for this Gene ID
UniProt
View information about B7-H1 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 1    Revision Date: 10/26/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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