RBC Lysis/Fixation Solution (10X)

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Other Names
Red blood cell lysing solution
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RBClysis_422401_1_092111
Human peripheral blood cells were stained with anti-human CD3 PerCP/Cy5.5 and anti-human CD4 FITC; red blood cells were lysed with BioLegend RBC Lysis/Fixation Solution.
  • RBClysis_422401_1_092111
    Human peripheral blood cells were stained with anti-human CD3 PerCP/Cy5.5 and anti-human CD4 FITC; red blood cells were lysed with BioLegend RBC Lysis/Fixation Solution.
  • RBClysis_422401_2_092111
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422401 50 ml $120
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Description

BioLegend RBC Lysis/Fixation Solution has been designed and optimized for one-step lysing of red blood cells (RBCs) and fixation  of the remaining blood elements (such as leukocytes) following immunofluorescent staining with fluorochrome-conjugated antibodies. It can be used for either lyse/wash or lyse/no wash immunofluorescent staining procedures. This solution is supplied as a 10X concentrate. Dilute to 1X working solution with room temperature dI H20 prior to use.

Product Details
Technical data sheet

Product Details

Formulation
This RBC Lysis/Fixation Solution is supplied as a 10X solution and should be diluted to 1X in room temperature deionized water.
Preparation
The pH of the 1X solution should fall within the range of pH 7.1 - 7.4. Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use.
Storage & Handling
Store RBC lysis buffer between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage


Whole Blood Lyse/Wash Procedure:

1. Prepare 1X RBC Lysis/Fixation solution by diluting 1 part of BioLegend RBC Lysis/Fixation solution with 9 parts of room temperature dI H20.
2. Mix 100 µl of whole blood with fluorochrome-conjugated antibody.
3. Incubate at room temperature, in the dark, for 20 minutes.
4. Add 2.0 mL 1X RBC Lysis/Fixation solution, gently mixing each tube immediately.
5. Incubate at room temperature, in the dark, for 15 minutes.
6. Centrifuge at 350Xg for five minutes.  Discard supernatant.
7. Add 2 mL BioLegend Cell Staining Buffer (Cat No 420201).
8. Centrifuge at 350Xg for five minutes. Discard supernatant.
9. Resuspend cell pellet in 0.3-0.5 mL Cell Staining Buffer.
10. Analyze cells immediately by flow cytometry or store at 4°C, in the dark, for no longer than 12 hours.

Whole Blood Lyse/No Wash Procedure:

1. Prepare 1X RBC Lysis/Fixation solution by diluting 1 part of BioLegend RBC Lysis/Fixation solution with 9 parts room temperature dI H20.
2. Mix 50 µl of whole blood with fluorochrome-conjugated antibody.
3. Incubate at room temperature, in the dark, for 20 minutes.
4. Add 0.45 mL 1X RBC Lysis/Fixation solution, gently mixing each tube immediately.
5. Incubate at room temperature, in the dark, for 15 minutes.
6. Analyze samples by flow cytometry.

Application Notes

The 1X Lysis/Fixation solution can be used within 1 month from the date of preparation. It can be stored at room temperature or between 2°C and 8°C.

Application References

(PubMed link indicates BioLegend citation)
  1. Horvath P, et al. 2012. J Infect Dis. PubMed
Product Citations
  1. Horváth P, et al. 2012. J Infect Dis. 205:923. PubMed
  2. Kusner L, et al. 2014. PLoS One. 9:102231. PubMed
  3. Javed A, et al. 2015. PLoS One. 10: 0142086. PubMed
  4. Foster G, et al. 2015. J Immunol. 195: 5380 - 5392. PubMed
  5. Nerdal P, et al. 2016. J Immunol. 197: 3059 - 3068. PubMed
  6. Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
  7. Curry A, et al. 2017. PLoS One. 12(2):e0171586. PubMed
  8. Camponova P, et al. 2017. Can J Physiol Pharmacol. 10.1139/cjpp-2016-0710. PubMed
  9. Lee J, et al. 2017. Biomaterials. 10.1016/j.biomaterials.2017.07.014. PubMed
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Antigen Details

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Go To Top Version: 2    Revision Date: 10/17/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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